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. 1990 Oct;94(2):752–759. doi: 10.1104/pp.94.2.752

Effect of Nitrogen Starvation on Polypeptide Composition, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase, and Thylakoid Carotenoprotein Content of Synechocystis sp. Strain PCC6308 1

Clifford S Duke 1,2, Mary M Allen 1
PMCID: PMC1077295  PMID: 16667775

Abstract

Synechocystis sp. strain PCC6308 cells were starved for nitrogen for 5 days. The polypeptide compositions of whole cell extracts and washed membranes of nitrogen-replete and nitrogen-starved cells were compared by one- and two-dimensional electrophoresis. Immunoblotting of one-dimensional gels indicated that pelletable ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) was depleted in cells starved for nitrogen, while levels of soluble Rubisco were comparable in nitrogen-starved and nitrogen-replete cells. This is consistent with the hypothesis that pelletable Rubisco may serve as a nitrogen reserve in Synechocystis 6308. Other polypeptides were differentially enriched in the membrane or soluble fractions of nitrogen-replete cells or nitrogen-starved cells, suggesting nitrogen starvation may alter partitioning of polypeptides into soluble and membrane fractions. Degradation of abundant polypeptides during nitrogen starvation appeared to cause an effective magnification of less abundant polypeptides in the molecular mass range of 20 to 40 kilodaltons, as shown by two-dimensional electrophoresis. A 42-kilodalton thylakoid carotenoid protein identified by immunoblotting was conserved in membranes from nitrogen-starved cells. This may be functional for cells depleted of pigment and thus exposed to higher light levels because of decreased self-shading.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen M. M. Cyanobacterial cell inclusions. Annu Rev Microbiol. 1984;38:1–25. doi: 10.1146/annurev.mi.38.100184.000245. [DOI] [PubMed] [Google Scholar]
  2. Allen M. M. Photosynthetic membrane system in Anacystis nidulans. J Bacteriol. 1968 Sep;96(3):836–841. doi: 10.1128/jb.96.3.836-841.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Allen M. M., Smith A. J. Nitrogen chlorosis in blue-green algae. Arch Mikrobiol. 1969;69(2):114–120. doi: 10.1007/BF00409755. [DOI] [PubMed] [Google Scholar]
  4. Borbély G., Surányi G., Korcz A., Pálfi Z. Effect of heat shock on protein synthesis in the cyanobacterium Synechococcus sp. strain PCC 6301. J Bacteriol. 1985 Mar;161(3):1125–1130. doi: 10.1128/jb.161.3.1125-1130.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bricker T. M., Sherman L. A. Triton X-114 phase fractionation of membrane proteins of the cyanobacterium Anacystis nidulans R2. Arch Biochem Biophys. 1984 Nov 15;235(1):204–211. doi: 10.1016/0003-9861(84)90269-8. [DOI] [PubMed] [Google Scholar]
  6. Duke C. S., Cezeaux A., Allen M. M. Changes in polypeptide composition of Synechocystis sp. strain 6308 phycobilisomes induced by nitrogen starvation. J Bacteriol. 1989 Apr;171(4):1960–1966. doi: 10.1128/jb.171.4.1960-1966.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Green L. S., Grossman A. R. Changes in sulfate transport characteristics and protein composition of Anacystis nidulans R2 during sulfur deprivation. J Bacteriol. 1988 Feb;170(2):583–587. doi: 10.1128/jb.170.2.583-587.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Groat R. G., Schultz J. E., Zychlinsky E., Bockman A., Matin A. Starvation proteins in Escherichia coli: kinetics of synthesis and role in starvation survival. J Bacteriol. 1986 Nov;168(2):486–493. doi: 10.1128/jb.168.2.486-493.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Masamoto K., Riethman H. C., Sherman L. A. Isolation and Characterization of a Carotenoid-Associated Thylakoid Protein from the Cyanobacterium Anacystis nidulans R2. Plant Physiol. 1987 Jul;84(3):633–639. doi: 10.1104/pp.84.3.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McNeil P. H., Walker D. A. The effect of magnesium and other ions on the distribution of ribulose 1,5-bisphosphate carboxylase in chloroplast extracts. Arch Biochem Biophys. 1981 Apr 15;208(1):184–188. doi: 10.1016/0003-9861(81)90138-7. [DOI] [PubMed] [Google Scholar]
  12. Miller L. S., Holt S. C. Effect of carbon dioxide on pigment and membrane content in Synechococcus lividus. Arch Microbiol. 1977 Nov 18;115(2):185–198. doi: 10.1007/BF00406374. [DOI] [PubMed] [Google Scholar]
  13. Morrissey J. H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. doi: 10.1016/0003-2697(81)90783-1. [DOI] [PubMed] [Google Scholar]
  14. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  15. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
  16. Sherman D. M., Sherman L. A. Effect of iron deficiency and iron restoration on ultrastructure of Anacystis nidulans. J Bacteriol. 1983 Oct;156(1):393–401. doi: 10.1128/jb.156.1.393-401.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. de Vasconcelos L., Fay P. Nitrogen metabolism and ultrastructure in Anabaena cylindrica. I. The effect of nitrogen starvation. Arch Mikrobiol. 1974 Mar 28;96(4):271–279. [PubMed] [Google Scholar]

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