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. 1990 Nov;94(3):882–886. doi: 10.1104/pp.94.3.882

An H+-ATPase Assay: Proton Pumping and ATPase Activity Determined Simultaneously in the Same Sample 1

Michael Gjedde Palmgren 1
PMCID: PMC1077317  PMID: 16667867

Abstract

A continuous spectrophotometric assay of H+-ATPase activity was developed by combining two well-known methods for measuring proton pumping and ATPase activity. Proton uptake into plasma membrane vesicles from Avena sativa L. (cv Rhiannon) was monitored as the absorbance decrease at 495 nm of the ΔpH probe acridine orange. Simultaneously, ATPase activity was measured by following the absorbance decrease at 340 nanometers by coupling ATP hydrolysis enzymatically to the oxidation of NADH. This H+-ATPase assay is convenient for determining the relative relationship between ATP hydrolysis and proton pumping.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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