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. 1990 Nov;94(3):1444–1448. doi: 10.1104/pp.94.3.1444

Biosynthesis of the Phytoalexin Pisatin 1

Isoflavone Reduction and Further Metabolism of the Product Sophorol by Extracts of Pisum sativum

Carol L Preisig 1,2,2, John N Bell 1,2,3, Yuejin Sun 1,2, Geza Hrazdina 1,2, David E Matthews 1,2,4, Hans D VanEtten 1,2,5
PMCID: PMC1077396  PMID: 16667851

Abstract

NADPH-dependent reduction of 2′,7-dihydroxy-4′,5′-methylenedioxyisoflavone to the isoflavanone sophorol, a proposed intermediate step in pisatin biosynthesis, was detected in extracts of Pisum sativum. This isoflavone reductase activity was inducible by treatment of pea seedlings with CuCl2. The timing of induction coincided with that of the 6a-hydroxymaackiain 3-O-methyltransferase, which catalyzes the terminal biosynthetic step. Neither enzyme was light inducible. Further NADPH-dependent metabolism of sophorol by extracts of Cucl2-treated seedlings was also observed; three products were radiolabeled when [3H]sophorol was the substrate, one of which is tentatively identified as maackiain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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