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. 1990 Nov;94(3):1488–1491. doi: 10.1104/pp.94.3.1488

Isolation of a cDNA Clone for α-Amylase in Mung Bean Cotyledons 1

Analysis of α-Amylase mRNA Levels in Cotyledons during and following Germination of Mung Bean Seeds

Nobuya Koizuka 1,2, Yoshiyuki Tanaka 1,2, Yukio Morohashi 1,2
PMCID: PMC1077404  PMID: 16667859

Abstract

A cDNA was isolated that codes for α-amylase in mung bean (Vigna radiata) cotyledons, and the nucleotide sequence was determined. The deduced amino acid sequence (421 amino acid residues) is about 65% homologous with those of barley α-amylases. By comparing the deduced sequence with the sequence of the purified α-amylase, it was inferred that 23 N-terminal amino acids of a nascent polypeptide represent a signal peptide. Northern blot analysis showed that the levels of α-amylase mRNA are in parallel with the activities of α-amylase synthesis in cotyledons. Under the conditions where the solute leakage from cotyledons is accelerated during imbibition, a rapid increase in the amount of the α-amylase mRNA occurs. We postulate that a factor(s) which regulates in an inhibitory manner the α-amylase expression at the transcriptional level may be present in dry cotyledons and be removed by leakage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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