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. 1990 Dec;94(4):1820–1829. doi: 10.1104/pp.94.4.1820

Short-Term Treatment with Cell Wall Degrading Enzymes Increases the Activity of the Inositol Phospholipid Kinases and the Vanadate-Sensitive ATPase of Carrot Cells 1

Qiuyun Chen 1, Wendy F Boss 1
PMCID: PMC1077459  PMID: 16667922

Abstract

Treating carrot (Daucus carota L.) suspension culture cells with a mixture of cell wall degrading enzymes, Driselase, resulted in an increase in the percentage of [3H]phosphatidylinositol bisphosphate. Analysis of the lipid kinase activities in the isolated plasma membranes after whole cell treatment indicated that treatment with Driselase (2% weight/volume; the equivalent of 340 units per milliliter of hemicellulase and 400 units per milliliter of cellulase activity) or treatment with hemicellulase (31.7% weight/volume, 20.7 units per milliliter) resulted in an increase in the inositol phospholipid kinase activity. However, treatment with cellulase alone had no effect at 0.5% (weight/volume, 17.2 units per milliliter) or inhibited the kinase activity at 1% (weight/volume, 34.4 units per milliliter). The active stimulus in Driselase was heat sensitive. The plasma membrane vanadate-sensitive ATPase activity also increased when the cells were treated with Driselase. A time course study indicated that both the inositol phospholipid kinases and the plasma membrane vanadate-sensitive ATPase responded to as little as 5 seconds of treatment with 2% Driselase. However, at the lowest concentration of Driselase (0.04%, weight/volume) that resulted in an increase in inositol phospholipid kinase activity, the ATPase activity was not affected. Because inositol phospholipids have been shown to activate the vanadate-sensitive ATPase from plants (AR Memon, Q Chen, WF Boss [1989] Biochem Biophys Res Commun 162: 1295-1301), a stimulus-response pathway involving both the inositol phospholipid kinases and the plasma membrane vanadate-sensitive ATPase activity is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albersheim P., Valent B. S. Host-pathogen interactions in plants. Plants, when exposed to oligosaccharides of fungal origin, defend themselves by accumulating antibiotics. J Cell Biol. 1978 Sep;78(3):627–643. doi: 10.1083/jcb.78.3.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blowers D. P., Boss W. F., Trewavas A. J. Rapid Changes in Plasma Membrane Protein Phosphorylation during Initiation of Cell Wall Digestion. Plant Physiol. 1988 Feb;86(2):505–509. doi: 10.1104/pp.86.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boss W. F., Massel M. O. Polyphosphoinositides are present in plant tissue culture cells. Biochem Biophys Res Commun. 1985 Nov 15;132(3):1018–1023. doi: 10.1016/0006-291x(85)91908-4. [DOI] [PubMed] [Google Scholar]
  4. Chauhan V. P., Brockerhoff H. Phosphatidylinositol-4,5-bisphosphate may antecede diacylglycerol as activator of protein kinase C. Biochem Biophys Res Commun. 1988 Aug 30;155(1):18–23. doi: 10.1016/s0006-291x(88)81043-x. [DOI] [PubMed] [Google Scholar]
  5. Choquette D., Hakim G., Filoteo A. G., Plishker G. A., Bostwick J. R., Penniston J. T. Regulation of plasma membrane Ca2+ ATPases by lipids of the phosphatidylinositol cycle. Biochem Biophys Res Commun. 1984 Dec 28;125(3):908–915. doi: 10.1016/0006-291x(84)91369-x. [DOI] [PubMed] [Google Scholar]
  6. Drøbak B. K., Ferguson I. B., Dawson A. P., Irvine R. F. Inositol-containing lipids in suspension-cultured plant cells: an isotopic study. Plant Physiol. 1988 May;87(1):217–222. doi: 10.1104/pp.87.1.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Einspahr K. J., Peeler T. C., Thompson G. A. Phosphatidylinositol 4,5-Bisphosphate Phospholipase C and Phosphomonoesterase in Dunaliella salina Membranes. Plant Physiol. 1989 Jul;90(3):1115–1120. doi: 10.1104/pp.90.3.1115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gallagher S. R., Leonard R. T. Effect of vanadate, molybdate, and azide on membrane-associated ATPase and soluble phosphatase activities of corn roots. Plant Physiol. 1982 Nov;70(5):1335–1340. doi: 10.1104/pp.70.5.1335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hahn M. G., Darvill A. G., Albersheim P. Host-Pathogen Interactions : XIX. THE ENDOGENOUS ELICITOR, A FRAGMENT OF A PLANT CELL WALL POLYSACCHARIDE THAT ELICITS PHYTOALEXIN ACCUMULATION IN SOYBEANS. Plant Physiol. 1981 Nov;68(5):1161–1169. doi: 10.1104/pp.68.5.1161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Joseph S. K., Esch T., Bonner W. D., Jr Hydrolysis of inositol phosphates by plant cell extracts. Biochem J. 1989 Dec 15;264(3):851–856. doi: 10.1042/bj2640851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kiehl R., Varsányi M., Neumann E. Phosphorylation of phosphatidylinositol associated with the nicotinic acetylcholine receptor of Torpedo californica. Biochem Biophys Res Commun. 1987 Sep 30;147(3):1251–1258. doi: 10.1016/s0006-291x(87)80205-x. [DOI] [PubMed] [Google Scholar]
  12. Kraft A. S., Anderson W. B. Phorbol esters increase the amount of Ca2+, phospholipid-dependent protein kinase associated with plasma membrane. Nature. 1983 Feb 17;301(5901):621–623. doi: 10.1038/301621a0. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lin S. H., Fain J. N. Ca2+-Mg2+-ATPase in rat hepatocyte plasma membranes: inhibition by vasopressin and purification of the enzyme. Prog Clin Biol Res. 1984;168:25–30. [PubMed] [Google Scholar]
  15. Lipsky J. J., Lietman P. S. Neomycin inhibition of adenosine triphosphatase: evidence for a neomycin-phospholipid interaction. Antimicrob Agents Chemother. 1980 Oct;18(4):532–535. doi: 10.1128/aac.18.4.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Memon A. R., Boss W. F. Rapid light-induced changes in phosphoinositide kinases and H(+)-ATPase in plasma membrane of sunflower hypocotyls. J Biol Chem. 1990 Sep 5;265(25):14817–14821. [PubMed] [Google Scholar]
  17. Memon A. R., Chen Q. Y., Boss W. F. Inositol phospholipids activate plasma membrane ATPase in plants. Biochem Biophys Res Commun. 1989 Aug 15;162(3):1295–1301. doi: 10.1016/0006-291x(89)90814-0. [DOI] [PubMed] [Google Scholar]
  18. Memon A. R., Rincon M., Boss W. F. Inositol Trisphosphate Metabolism in Carrot (Daucus carota L.) Cells. Plant Physiol. 1989 Oct;91(2):477–480. doi: 10.1104/pp.91.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Morré D. J., Pfaffmann H., Drobes B., Wilkinson F. E., Hartmann E. Diacylglycerol Levels Unchanged during Auxin-Stimulated Growth of Excised Hypocotyl Segments of Soybean. Plant Physiol. 1989 May;90(1):275–279. doi: 10.1104/pp.90.1.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Morse M. J., Crain R. C., Satter R. L. Light-stimulated inositolphospholipid turnover in Samanea saman leaf pulvini. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7075–7078. doi: 10.1073/pnas.84.20.7075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nishioka H., Imoto M., Sawa T., Hamada M., Naganawa H., Takeuchi T., Umezawa K. Screening of phosphatidylinositol kinase inhibitors from Streptomyces. J Antibiot (Tokyo) 1989 May;42(5):823–825. doi: 10.7164/antibiotics.42.823. [DOI] [PubMed] [Google Scholar]
  22. Rincón M., Chen Q., Boss W. F. Characterization of Inositol Phosphates in Carrot (Daucus carota L.) Cells. Plant Physiol. 1989 Jan;89(1):126–132. doi: 10.1104/pp.89.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rogers K. R., Albert F., Anderson A. J. Lipid peroxidation is a consequence of elicitor activity. Plant Physiol. 1988 Feb;86(2):547–553. doi: 10.1104/pp.86.2.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. TAUSSKY H. H., SHORR E. A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem. 1953 Jun;202(2):675–685. [PubMed] [Google Scholar]
  25. Wheeler J. J., Boss W. F. Polyphosphoinositides are present in plasma membranes isolated from fusogenic carrot cells. Plant Physiol. 1987 Oct;85(2):389–392. doi: 10.1104/pp.85.2.389. [DOI] [PMC free article] [PubMed] [Google Scholar]

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