Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1991 Feb;95(2):390–398. doi: 10.1104/pp.95.2.390

Oxidative Phosphorylation by Mitochondria Extracted from Dry Sunflower Seeds

Sylvie Attucci 1,2, Jean Pierre Carde 1,2, Philippe Raymond 1,2, Véronique Saint-Gès 1,2, Anne Spiteri 1,2, Alain Pradet 1,2
PMCID: PMC1077543  PMID: 16667996

Abstract

The role of mitochondria in the phosphorylation of ADP to ATP in the early steps of seed germination has been studied. Mitochondria were extracted from dry sunflower (Helianthus annuus) seeds. Adenylate kinase-dependent ATP synthesis was inhibited by p1,p5-di(adenosine-5′)pentaphosphate. Synthesis of ATP was observed with the different substrates: citrate, α-ketoglutarate, succinate, malate, pyruvate or NADH. This synthesis was activated by cytochrome c, and inhibited by cyanide, oligomycin, carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone, and carboxyatractyloside. The ATP/O values with succinate were 0.85 and 1.2 in the absence or presence, respectively, of cytochrome c. Electron micrographs showed that mitochondria of dry tissues have different structures when observed in situ or in vitro after aqueous extraction, suggesting that profound changes occurred after the contact with the aqueous medium. These results confirm previous data obtained in vivo showing that mitochondria present in dry seeds are able to synthesize ATP as soon as the seeds are rehydrated.

Full text

PDF
390

Images in this article

395Figure 7
on p.395

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crowe J. H., Crowe L. M., Carpenter J. F., Aurell Wistrom C. Stabilization of dry phospholipid bilayers and proteins by sugars. Biochem J. 1987 Feb 15;242(1):1–10. doi: 10.1042/bj2420001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Douce R., Christensen E. L., Bonner W. D., Jr Preparation of intaintact plant mitochondria. Biochim Biophys Acta. 1972 Aug 17;275(2):148–160. doi: 10.1016/0005-2728(72)90035-7. [DOI] [PubMed] [Google Scholar]
  3. Duke S. H., Kakefuda G., Harvey T. M. Differential leakage of intracellular substances from imbibing soybean seeds. Plant Physiol. 1983 Aug;72(4):919–924. doi: 10.1104/pp.72.4.919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ehrenshaft M., Brambl R. Respiration and mitochondrial biogenesis in germinating embryos of maize. Plant Physiol. 1990 May;93(1):295–304. doi: 10.1104/pp.93.1.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hourmant A., Pradet A. Oxidative Phosphorylation in Germinating Lettuce Seeds (Lactuca sativa) during the First Hours of Imbibition. Plant Physiol. 1981 Sep;68(3):631–635. doi: 10.1104/pp.68.3.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johnson K. D., Herman E. M., Chrispeels M. J. An abundant, highly conserved tonoplast protein in seeds. Plant Physiol. 1989 Nov;91(3):1006–1013. doi: 10.1104/pp.91.3.1006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lienhard G. E., Secemski I. I. P 1 ,P 5 -Di(adenosine-5')pentaphosphate, a potent multisubstrate inhibitor of adenylate kinase. J Biol Chem. 1973 Feb 10;248(3):1121–1123. [PubMed] [Google Scholar]
  8. Loomis W. D. Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol. 1974;31:528–544. doi: 10.1016/0076-6879(74)31057-9. [DOI] [PubMed] [Google Scholar]
  9. Morohashi Y., Bewley J. D., Yeung E. C. Biogenesis of Mitochondria in Imbibed Peanut Cotyledons : II. DEVELOPMENT OF LIGHT AND HEAVY MITOCHONDRIA. Plant Physiol. 1981 Aug;68(2):318–323. doi: 10.1104/pp.68.2.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Neuburger M., Journet E. P., Bligny R., Carde J. P., Douce R. Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll. Arch Biochem Biophys. 1982 Aug;217(1):312–323. doi: 10.1016/0003-9861(82)90507-0. [DOI] [PubMed] [Google Scholar]
  11. Raymond P., Al-Ani A., Pradet A. ATP Production by Respiration and Fermentation, and Energy Charge during Aerobiosis and Anaerobiosis in Twelve Fatty and Starchy Germinating Seeds. Plant Physiol. 1985 Nov;79(3):879–884. doi: 10.1104/pp.79.3.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Singer T. P., Oestreicher G., Hogue P. Regulation of Succinate Dehyrogenase in Higher Plants: I. Some General Characteristics of the Membrane-bound Enzyme. Plant Physiol. 1973 Dec;52(6):616–621. doi: 10.1104/pp.52.6.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vertucci C. W., Leopold A. C. Bound water in soybean seed and its relation to respiration and imbibitional damage. Plant Physiol. 1984 May;75(1):114–117. doi: 10.1104/pp.75.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wolk W. D., Dillon P. F., Copeland L. F., Dilley D. R. Dynamics of Imbibition in Phaseolus vulgaris L. in Relation to Initial Seed Moisture Content. Plant Physiol. 1989 Mar;89(3):805–810. doi: 10.1104/pp.89.3.805. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES