Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1991 Feb;95(2):648–651. doi: 10.1104/pp.95.2.648

Drought Stress, Enzymes of Glutathione Metabolism, Oxidation Injury, and Protein Synthesis in Tortula ruralis1

Rajinder S Dhindsa 1
PMCID: PMC1077581  PMID: 16668032

Abstract

The activities of glutathione reductase (EC 1.6.4.2), glutathione peroxidase (EC 1.11.1.9), and glutathione S-transferase (EC 2.5.1.18) were found to increase during slow drying or during rehydration following rapid drying of the drought-tolerant moss Tortula ruralis. Little change was observed in the activity of malate deydrogenase (NAD+ oxidoreductase, EC 1.1.1.37) during dehydration or subsequent rehydration. When the tissue was treated with cycloheximide, actinomycin D, or cordycepin, the increase in the activities of glutathione reductase and glutathione S-transferase was largely prevented while effect on glutathione peroxidase was much smaller. Concomitantly, oxidized glutathione (GSSG) as percentage of total glutathione increased. GSSG level was correlated positively with the levels of lipid peroxidation and solute leakage and negatively with the rate of protein synthesis. The results show that GSSG level is a good indicator of oxidation stress and provide support to the suggestion that GSSG mediates, at least in part, the drought stress-induced inhibition of protein synthesis.

Full text

PDF
648

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson M. E. Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol. 1985;113:548–555. doi: 10.1016/s0076-6879(85)13073-9. [DOI] [PubMed] [Google Scholar]
  2. Carlberg I., Mannervik B. Glutathione reductase. Methods Enzymol. 1985;113:484–490. doi: 10.1016/s0076-6879(85)13062-4. [DOI] [PubMed] [Google Scholar]
  3. Dhindsa R. S., Bewley J. D. Plant desiccation: polysome loss not due to ribonuclease. Science. 1976 Jan 16;191(4223):181–182. doi: 10.1126/science.1246604. [DOI] [PubMed] [Google Scholar]
  4. Dhindsa R. S., Bewley J. D. Water Stress and Protein Synthesis: V. Protein Synthesis, Protein Stability, and Membrane Permeability in a Drought-sensitive and a Drought-tolerant Moss. Plant Physiol. 1977 Feb;59(2):295–300. doi: 10.1104/pp.59.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dhindsa R. S. Glutathione Status and Protein Synthesis during Drought and Subsequent Rehydration in Tortula ruralis. Plant Physiol. 1987 Apr;83(4):816–819. doi: 10.1104/pp.83.4.816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dhindsa R. S. Protein Synthesis during Rehydration of Rapidly Dried Tortula ruralis: Evidence for Oxidation Injury. Plant Physiol. 1987 Dec;85(4):1094–1098. doi: 10.1104/pp.85.4.1094. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ernst V., Levin D. H., London I. M. Inhibition of protein synthesis initiation by oxidized glutathione: activation of a protein kinase that phosphorylates the alpha subunit of eukaryotic initiation factor 2. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4110–4114. doi: 10.1073/pnas.75.9.4110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Habig W. H., Jakoby W. B. Assays for differentiation of glutathione S-transferases. Methods Enzymol. 1981;77:398–405. doi: 10.1016/s0076-6879(81)77053-8. [DOI] [PubMed] [Google Scholar]
  9. Hochstein P., Utley H. Hydrogen peroxide detoxication by glutathione peroxidase and catalase in rat liver homogenates. Mol Pharmacol. 1968 Nov;4(6):574–579. [PubMed] [Google Scholar]
  10. Kosower N. S., Vanderhoff G. A., Benerofe B., Hunt T., Kosower E. M. Inhibition of protein synthesis by glutathione disulfide in the presence of glutathione. Biochem Biophys Res Commun. 1971 Nov 5;45(3):816–821. doi: 10.1016/0006-291x(71)90490-6. [DOI] [PubMed] [Google Scholar]
  11. Meister A. Selective modification of glutathione metabolism. Science. 1983 Apr 29;220(4596):472–477. doi: 10.1126/science.6836290. [DOI] [PubMed] [Google Scholar]
  12. Sevanian A., Muakkassah-Kelly S. F., Montestruque S. The influence of phospholipase A2 and glutathione peroxidase on the elimination of membrane lipid peroxides. Arch Biochem Biophys. 1983 Jun;223(2):441–452. doi: 10.1016/0003-9861(83)90608-2. [DOI] [PubMed] [Google Scholar]
  13. Ting I. P. Malic dehydrogenases in corn root tips. Arch Biochem Biophys. 1968 Jul;126(1):1–7. doi: 10.1016/0003-9861(68)90552-3. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES