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. 1991 Mar;95(3):760–767. doi: 10.1104/pp.95.3.760

Response to Anoxia in Rice and Wheat Seedlings

Changes in the pH of Intracellular Compartments, Glucose-6-Phosphate Level, and Metabolic Rate

Faustino Menegus 1,2,3, Liliana Cattaruzza 1,2,3, Monica Mattana 1,2,3, Nicoletta Beffagna 1,2,3, Enzio Ragg 1,2,3
PMCID: PMC1077603  PMID: 16668051

Abstract

31P nuclear magnetic resonance spectroscopy was used to measure intracellular pH in living tissues. Oxygen deprivation caused fast cytoplasmic acidification from pH 7.4 to 7.0 in shoots of rice, Oryza sativa L. var arborio, a species highly resistant to anoxia. Acidification was complete after 10 minutes of anoxia. Alkalinization of both cytosplasm and vacuole followed thereafter. In the anoxia intolerant wheat shoots, Triticum aestivum L. var MEK, the same treatment caused a sharper cytoplasmic acidification, from pH 7.4 to 6.6, which occurred during a period of 2 hours. Cytoplasmic acidification continued with progress of anoxia and there was no vacuolar alkalinization comparable to the one observed in rice. In wheat oxyen, withdrawal also caused the reduction of both glucose-6-phosphate level and of metabolic rate. It also induced heavy losses of inorganic phosphate from tissues. Conversely, in rice, glucose-6-phosphate level and metabolic rate were increased and inorganic phosphate leakage from tissues was completely absent. These results are discussed in relation to the mechanisms of plant resistance to anoxia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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