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. 1991 Mar;95(3):832–838. doi: 10.1104/pp.95.3.832

Osmosensitivity of Sucrose Uptake by Immature Pea Cotyledons Disappears during Development

Frank C Lanfermeijer 1, Judith W Koerselman-Kooij 1, Adrianus C Borstlap 1
PMCID: PMC1077613  PMID: 16668061

Abstract

Sucrose uptake was studied in isolated, immature pea cotyledons (Pisum sativum L. cv Marzia) in relation to their developmental stage. During the developmental period examined the water content of the cotyledons decreased from ≈80% “stage 1” to ≈55% “stage 2”. When assayed in an isotonic medium (400 osmoles per cubic meter) the influx capacity per gram fresh weight for sucrose was almost constant during this developmental period. The influx could be analyzed into a saturable component (Km ≈ 9 moles per cubic meter; Vmax ≈ 150 nanomoles per minute per gram fresh weight) and an unsaturable component (ki ≈ 0.5 nanomoles per minute per gram fresh weight [per mole per cubic meter]). Incubation in a hypotonic medium reduced the sucrose influx in stage 1 cotyledons, up to 80% reduction at 0 milliosmole (medium without mannitol), but had no effect on sucrose uptake by stage 2 cotyledons. Reduced uptake in a hypotonic medium (100 osmoles per cubic meter) could be attributed to a lowering of the Vmax from 150 to 36 nanomoles per minute per gram fresh weight. During incubation of stage 1 cotyledons and stage 2-cotyledons in a hypotonic medium (200 osmoles per cubic meter) their volume increased by 16% and 5.6%, respectively, while the calculated turgor pressure increased from 0.2 to 0.6 megapascal for cotyledons of both developmental stages. Reduced sucrose influx in hypotonic medium, therefore, seems to be related to cell swelling (membrane stretching) rather than to increased turgor pressure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett A. B., Spanswick R. M. Derepression of amino Acid-h cotransport in developing soybean embryos. Plant Physiol. 1983 Jul;72(3):781–786. doi: 10.1104/pp.72.3.781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gifford R. M., Thorne J. H. Sucrose Concentration at the Apoplastic Interface between Seed Coat and Cotyledons of Developing Soybean Seeds. Plant Physiol. 1985 Apr;77(4):863–868. doi: 10.1104/pp.77.4.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kinraide T. B., Wyse R. E. Electrical evidence for turgor inhibition of proton extrusion in sugar beet taproot. Plant Physiol. 1986 Dec;82(4):1148–1150. doi: 10.1104/pp.82.4.1148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Li Z. S., Delrot S. Osmotic dependence of the transmembrane potential difference of broadbean mesocarp cells. Plant Physiol. 1987 Jul;84(3):895–899. doi: 10.1104/pp.84.3.895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lichtner F. T., Spanswick R. M. Electrogenic sucrose transport in developing soybean cotyledons. Plant Physiol. 1981 Apr;67(4):869–874. doi: 10.1104/pp.67.4.869. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lichtner F. T., Spanswick R. M. Sucrose uptake by developing soybean cotyledons. Plant Physiol. 1981 Sep;68(3):693–698. doi: 10.1104/pp.68.3.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Oren-Shamir M., Pick U., Avron M. Involvement of the Plasma Membrane ATPase in the Osmoregulatory Mechanism of the Alga Dunaliella salina. Plant Physiol. 1989 Apr;89(4):1258–1263. doi: 10.1104/pp.89.4.1258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Reinhold L., Seiden A., Volokita M. Is modulation of the rate of proton pumping a key event in osmoregulation? Plant Physiol. 1984 Jul;75(3):846–849. doi: 10.1104/pp.75.3.846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ripp K. G., Viitanen P. V., Hitz W. D., Franceschi V. R. Identification of membrane protein associated with sucrose transport into cells of developing soybean cotyledons. Plant Physiol. 1988 Dec;88(4):1435–1445. doi: 10.1104/pp.88.4.1435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saab I. N., Obendorf R. L. Soybean Seed Water Relations during in Situ and in Vitro Growth and Maturation. Plant Physiol. 1989 Feb;89(2):610–616. doi: 10.1104/pp.89.2.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Thorne J. H. Characterization of the active sucrose transport system of immature soybean embryos. Plant Physiol. 1982 Oct;70(4):953–958. doi: 10.1104/pp.70.4.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wyse R. E., Zamski E., Tomos A. D. Turgor regulation of sucrose transport in sugar beet taproot tissue. Plant Physiol. 1986 Jun;81(2):478–481. doi: 10.1104/pp.81.2.478. [DOI] [PMC free article] [PubMed] [Google Scholar]

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