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. 1991 Apr;95(4):1174–1180. doi: 10.1104/pp.95.4.1174

1-Naphthyl Acetate-Dependent Medium Acidification by Zea mays L. Coleoptile Segments 1

Julio Salguero 1, Angeles Calatayud 1, Francisco Gonzalez-Daros 1, Secundino del Valle-Tascon 1
PMCID: PMC1077669  PMID: 16668108

Abstract

Zea mays L. cv INRA 5a coleoptile segments ecidify the incubation medium on the addition of 1-naphthyl acetate (1-NA). The buffering capacity of the bathing solution increases during 1-NA stimulated medium acidification. The solution bathing the 1-NA treated coleoptile segment was analyzed by high performance liquid chromatography. A considerable amount of acetic acid was detected in the bathing solution used to measure 1-NA-dependent medium acidification. For the first time, the data demonstrate directly the release of acetic acid from 1-NA. The extent of medium acidification was proportional to 1-NA concentration. Simultaneous measurement of medium acidification and acetate content upon addition of 1-NA showed that both processes were temporally correlated. The stoichiometry of proton equivalents to acetate ion was 0.966. Addition of 50 micromolar N,N′-dicyclohexylcarbodiimide had little effect on 1-NA-dependent medium acidification. The results indicate that 1-NA is hydrolyzed in the extracellular space of coleoptile cells.

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Selected References

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