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. 1991 Apr;95(4):1229–1236. doi: 10.1104/pp.95.4.1229

Studies on the System Regulating Proton Movement across the Chloroplast Envelope 1

Effects of ATPase Inhibitors, Mg2+, and an Amine Anesthetic on Stromal pH and Photosynthesis

Jeanne S Peters 1,2, Gerald A Berkowitz 1,2
PMCID: PMC1077677  PMID: 16668116

Abstract

Studies were undertaken to further characterize the spinach (Spinacea oleracea) chloroplast envelope system, which facilitates H+ movement into and out of the stroma, and, hence, modulates photosynthetic activity by regulating stromal pH. It was demonstrated that high envelope-bound Mg2+ causes stromal acidification and photosynthetic inhibition. High envelope-bound Mg2+ was also found to necessitate the activity of a digitoxinand oligomycin-sensitive ATPase for the maintenance of high stromal pH and photosynthesis in the illuminated chloroplast. In chloroplasts that had high envelope Mg2+ and inhibited envelope ATPase activity, 2-(diethylamino)-N-(2,6-dimethylphenyl)acetamide was found to raise stromal pH and stimulate photosynthesis. 2-(Diethylamino)-N-(2,6-dimethylphenyl)acetamide is an amine anesthetic that is known to act as a monovalent cation channel blocker in mammalian systems. We postulate that the system regulating cation and H+ fluxes across the plastid envelope includes a monovalent cation channel in the envelope, some degree of (envelope-bound Mg2+ modulated) H+ flux linked to monovalent cation antiport, and ATPase-dependent H+ efflux.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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