Table 1.
Activity against amastigote forms of T. cruzi and cytotoxicity against mammalian (NCTC) cells of compounds 1–8, isolated from MeOH extract from aerial parts of B. sphenophylla.
| Compound |
T. cruzi Trypomastigote EC50 (μM) ± SD |
T. cruzi Amastigote EC50 (μM) ± SD |
NCTC Mammalian Cell CC50 (μM) ± SD |
T. cruzi Trypomastigote SI |
T. cruzi Amastigote SI |
|---|---|---|---|---|---|
| 1 | 20.1 ± 1.5 | NA | >200 | >10.0 | - |
| 2 | 2.9 ± 1.5 | NA | >200 | >68.7 | - |
| 3 | 10.6 ± 4.4 | NA | >200 | >18.8 | - |
| 4 | NA | 12.8 ± 0.3 | >200 | - | >12.6 |
| 5 | NA | 2.7 ± 0.2 | >200 | - | >74.1 |
| 6–8 | NA | 24.9 ± 1.3 | >200 | - | >8.0 |
| Benznidazole | 18.7 ± 4.1 | 5.5 ± 2.2 | >200 | >10.7 | >36.4 |
EC50—50% effective concentration; CC50—50% cytotoxic concentration; SI—selectivity index; NA—not active; SD—standard deviation; samples were tested in duplicate and the assays were repeated at least twice.