Fig. 1. Dars2^IEC-KO mice develop severe intestinal pathology with massive lipid accumulation within large LDs in enterocytes.

a,b, Kaplan–Meier survival curves (a) and body weight at the age of 7 days (b) of Dars2^fl/fl (n = 56 (a), n = 68 (b)) and Dars2^IEC-KO (n = 57 (a), n = 66 (b)) mice. c, Immunoblot of IEC protein extracts from 7-day-old Dars2^fl/fl (n = 3) and Dars2^IEC-KO (n = 3) pups with the indicated antibodies. β-actin was used as the loading control. d, Representative images of SI sections from Dars2^fl/fl and Dars2^IEC-KO mice stained with enzyme histochemical staining for COX and SDH. e, Representative transmission electron microscopy (TEM) micrographs of SI sections from 7-day-old Dars2^fl/fl and Dars2^IEC-KO mice (n = 3 per genotype). G, Golgi; M, mitochondria. f, Representative images of SI sections from Dars2^fl/fl and Dars2^IEC-KO mice stained with haematoxylin & eosin (H&E), ORO or immunostained for PLIN2 and Ki67. g,h, TAG species content in SI (g) and liver (h) of Dars2^fl/fl (n = 7) and Dars2^IEC-KO (n = 7 SI, n = 6 liver) mice. i, Concentration of glucose, total cholesterol, TAGs, HDL-cholesterol and LDL-cholesterol in sera from 7-day-old Dars2^fl/fl and Dars2^IEC-KO mice (n = 29 (glucose, total cholesterol) per genotype; n = 23 (HDL, LDL) per genotype; n = 28, n = 25 (TAG) for Dars2^fl/fl and Dars2^IEC-KO, respectively). In b,g–i, dots represent individual mice, bar graphs show the mean ± s.e.m. and P values were calculated using two-sided nonparametric Mann–Whitney U-test. In a, P values were calculated using two-sided Gehan–Breslow–Wilcoxon test. In d,f, histological images are representative of the number of mice analysed as indicated in Supplementary Table 4. In c, each lane represents one mouse. Scale bars, 1 μm (e) or 50 μm (d,f). For gel source data, see Supplementary Fig. 1.

Source Data