Figure S4.

Time course of oculomotor nerve development in human embryos, related to Figure 3

All panels are LSFM images of solvent-cleared embryos (A–C) and fetus (D) immunostained with anti-MHC combined with ChAT (A–C) or Synaptophysin (D). (A)–(D) are individual images illustrating the developmental time course of all extraocular muscles and their innervation between PCW5.6 and PCW11.3. The inset on the right gives the color code used for nerve and muscles. Scale bars: all panels are counted from left to right for each row; 100 μm in (A, first and third panels from the left) and (B, fourth and fifth panels); 300 μm in (A, second panel), (C, first, second, sixth, and seventh panels), and (D, first, second, and seventh panels); 200 μm in (A, fourth panel) (B, first and second panels), and (C, third, fourth, and fifth panels); 150 μm in (B, third and sixth panels); and 500 μm in (D, third, fourth, and sixth panels).