Medical Sciences Correction for “Deregulation of the Hippo pathway in soft-tissue sarcoma promotes FOXM1 expression and tumorigenesis,” by T. S. Karin Eisinger-Mathason, Vera Mucaj, Kevin M. Biju, Michael S. Nakazawa, Mercy Gohil, Timothy P. Cash, Sam S. Yoon, Nicolas Skuli, Kyung Min Park, Sharon Gerecht, and M. Celeste Simon, which was first published June 15, 2015; 10.1073/pnas.1420005112 (Proc. Natl. Acad. Sci. U.S.A. 112, E3402–E3411).
The authors note that Fig. 6 appeared incorrectly. The corrected figure and its legend appear below. The online version has been corrected.
Fig. 6.
Foxm1 is required for sarcomagenesis in vivo. (A) FOXM1 expression was inhibited in HT-1080 cells, and 1 × 106 cells were injected s.c. in a xenograft model. Reduction in FOXM1 expression significantly reduced tumor growth (n = 5 mice/10 tumors per group). *P < 0.05 (P < 0.04 at days 16, 18, and 20). (B) Tumor weight from xenografts in A was also decreased in FOXM1 knockdown cells. *P < 0.05 (P = 0.0091). (C) Autochthonous mouse model of UPS; KP was crossed with Foxm1fl/fl mice to create KPF animals. Foxm1 deletion significantly inhibited sarcomagenesis [n = 10 (KP); n = 15 (KPF); P = 0.0091]. (D) Tumor weight was dramatically decreased in KPF tumors compared with KP. *P < 0.05 (P = 0.0002). (E) Images of KP (Left) and KPF (Right) tumor-bearing mice. Arrow indicates tumor. (F) Genotyping of KP and KPF animals and tumors showing recombination at the Foxm1 locus in KPF tumors. (G) H&E images from KP and KPF tumors showing the prevalence of irregular and heterogeneous tumor cells in KP tumors, as well as the reduction in necrotic and stromal compartments due to Foxm1 deletion. (Scale bars: 100 μm.) (H) FOXM1 IHC and H&E of KP and KPF tumor sections showing that FOXM1 protein expression is lost in the small KPF tumors. Arrow indicates tumor. (Scale bar: 100 μm.)