. 2023 Dec 8;15(1):e02192-23. doi: 10.1128/mbio.02192-23

TABLE 1.

Sequences of primers used for Microsporidia MB detection, Anopheles species identification, and amplification of reference genes for relative quantification of Microsporidia MB

Target	Primer name	Primer sequence (5′–3′)	Annealing temp.
Microsporidia MB 18S gene	MB18S 181F (10)	CGCCGGCCGTGAAAAATTTA	62°C
Microsporidia MB 18S gene	MB18S 650R (10)	CCTTGGACGTGGGAGCTATC	62°C
An. gambiae s.s. short interspersed elements (SINEs) 200	SINE 200F (16)	TCGCCTTAGACCTTGCGTTA	56°C
An. gambiae s.s. short interspersed elements (SINEs) 200	SINE 200R (16)	CGCTTCAAGAATTCGAGATAC	56°C
Anopheles ribosomal S7	S7UniF (10)	TCCTGGAGCTGGAGATGAAC	62°C
Anopheles ribosomal S7	S7UniR (10)	GACGGGTCTGTACCTTCTGG	62°C
An. gambiae s.s. actin gene	Actin-F (this study)	AAGATGACGTGTTGTGCCCT	64°C
An. gambiae s.s. actin gene	Actin-R (this study)	ATTCCCGTTGCTGGAAGTGT	64°C
An. gambiae s.s. GAPDH gene	An. ga. GAPDH-Aste-QPCR-F (this study)	GCGGTGGGCAAGGTCATCCC	64°C
An. gambiae s.s. GAPDH gene	GAPDH-Aste-QPCR-R (17)	TTCATCGGTCCGTTGGCGGC	64°C