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[Preprint]. 2023 Dec 28:2023.12.27.573459. [Version 1] doi: 10.1101/2023.12.27.573459

Common genetic variation impacts stress response in the brain

Carina Seah, Rebecca Signer, Michael Deans, Heather Bader, Tom Rusielewicz, Emily M Hicks, Hannah Young, Alanna Cote, Kayla Townsley, Changxin Xu, Christopher J Hunter, Barry McCarthy, Jordan Goldberg, Saunil Dobariya, Paul E Holtzherimer, Keith A Young; NYSCF Global Stem Cell Array® Team; Traumatic Stress Brain Research Group, Scott A Noggle, John H Krystal, Daniel Paull, Matthew J Girgenti, Rachel Yehuda, Kristen J Brennand, Laura M Huckins
PMCID: PMC10793429  PMID: 38234801

ABSTRACT

To explain why individuals exposed to identical stressors experience divergent clinical outcomes, we determine how molecular encoding of stress modifies genetic risk for brain disorders. Analysis of post-mortem brain (n=304) revealed 8557 stress-interactive expression quantitative trait loci (eQTLs) that dysregulate expression of 915 eGenes in response to stress, and lie in stress-related transcription factor binding sites. Response to stress is robust across experimental paradigms: up to 50% of stress-interactive eGenes validate in glucocorticoid treated hiPSC-derived neurons (n=39 donors). Stress-interactive eGenes show brain region- and cell type-specificity, and, in post-mortem brain, implicate glial and endothelial mechanisms. Stress dysregulates long-term expression of disorder risk genes in a genotype-dependent manner; stress-interactive transcriptomic imputation uncovered 139 novel genes conferring brain disorder risk only in the context of traumatic stress. Molecular stress-encoding explains individualized responses to traumatic stress; incorporating trauma into genomic studies of brain disorders is likely to improve diagnosis, prognosis, and drug discovery.

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