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. 2024 Jan 12;15(1):2302246. doi: 10.1080/21655979.2024.2302246

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure displays functional data derived from dendritic cells showing that the purified CD40 nanobody variants from figure 5 with 6 and 9 nanobody copies are much more active than the variant with only 4 copies. — CD40 agonists with intrinsic agonism trigger maturation and activation of DCs. immature monocyte-derived dendritic cells (iDcs) were generated by cultivation of monocytes for 7 days with GM-CSF/IL4. (a) iDCs were treated with 200 ng/ml of the indicated constructs and were then analyzed after 2 days by flow cytometry for the cell surface expression of CD14 (left panel) and CD83 (right panel). (b) iDCs were treated with 200 ng/ml of the indicated constructs and were analyzed next day by western blotting for the presence of the indicated proteins. (c) iDCs were treated with 8, 40 or 200 ng/ml of the indicated constructs overnight and finally cell culture supernatants were analyzed for their IL8 content by ELISA. n.s. = non significant; *p < 0.05; **p < 0.01, ***p < 0.001.