Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1992 Jan;98(1):157–162. doi: 10.1104/pp.98.1.157

Differential Protein Accumulation in Banana Fruit during Ripening 1

Eva Dominguez-Puigjaner 1,2, Miguel Vendrell 1,2, M Dolors Ludevid 1,2
PMCID: PMC1080163  PMID: 16668607

Abstract

Banana (Musa acuminata, cv Dwarf Cavendish) proteins were extracted from pulp tissue at different stages of ripening and analyzed by two-dimensional electrophoresis. The results provide evidence of differential protein accumulation during ripening. Two sets of polypeptides have been detected that increase substantially in ripe fruit. These polypeptides were characterized as glycoproteins by western blotting and concanavalin A binding assays. Antibodies againts tomato polygalacturonase cross-react with one of these sets of proteins.

Full text

PDF
157

Images in this article

158Figure 1
on p.158
159Figure 2
on p.159
160Figure 3
on p.160
160Figure 4
on p.160

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clegg J. C. Glycoprotein detection in nitrocellulose transfers of electrophoretically separated protein mixtures using concanavalin A and peroxidase: application to arenavirus and flavivirus proteins. Anal Biochem. 1982 Dec;127(2):389–394. doi: 10.1016/0003-2697(82)90192-0. [DOI] [PubMed] [Google Scholar]
  2. De Leo P., Sacher J. A. Senescense: association of synthesis of Acid phosphatase with banana ripening. Plant Physiol. 1970 Aug;46(2):208–211. doi: 10.1104/pp.46.2.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dellapenna D., Alexander D. C., Bennett A. B. Molecular cloning of tomato fruit polygalacturonase: Analysis of polygalacturonase mRNA levels during ripening. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6420–6424. doi: 10.1073/pnas.83.17.6420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kanellis A. K., Solomos T., Mattoo A. K. Changes in Sugars, Enzymic Activities and Acid Phosphatase Isoenzyme Profiles of Bananas Ripened in Air or Stored in 2.5% O(2) with and without Ethylene. Plant Physiol. 1989 May;90(1):251–258. doi: 10.1104/pp.90.1.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kanellis A. K., Solomos T., Mattoo A. K. Hydrolytic Enzyme Activities and Protein Pattern of Avocado Fruit Ripened in Air and in Low Oxygen, with and without Ethylene. Plant Physiol. 1989 May;90(1):259–266. doi: 10.1104/pp.90.1.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Meyer Y., Chartier Y. Hormonal Control of Mitotic Development in Tobacco Protoplasts: TWO-DIMENSIONAL DISTRIBUTION OF NEWLY-SYNTHESIZED PROTEINS. Plant Physiol. 1981 Dec;68(6):1273–1278. doi: 10.1104/pp.68.6.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Moshrefi M., Luh B. S. Carbohydrate composition and electrophoretic properties of tomato polygalacturonase isoenzymes. Eur J Biochem. 1983 Oct 3;135(3):511–514. doi: 10.1111/j.1432-1033.1983.tb07681.x. [DOI] [PubMed] [Google Scholar]
  9. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  10. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
  11. Schuch W., Bird C. R., Ray J., Smith C. J., Watson C. F., Morris P. C., Gray J. E., Arnold C., Seymour G. B., Tucker G. A. Control and manipulation of gene expression during tomato fruit ripening. Plant Mol Biol. 1989 Sep;13(3):303–311. doi: 10.1007/BF00025318. [DOI] [PubMed] [Google Scholar]
  12. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tucker G. A., Robertson N. G., Grierson D. The conversion of tomato-fruit polygalacturonase isoenzyme 2 into isoenzyme 1 in vitro. Eur J Biochem. 1981 Mar 16;115(1):87–90. doi: 10.1111/j.1432-1033.1981.tb06201.x. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES