Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1992 Feb;98(2):525–529. doi: 10.1104/pp.98.2.525

Evaluation of the Extent of Homologous Chloroplast DNA Sequences in the Mitochondrial Genome of Cowpea (Vigna unguiculata L.) 1

Elena G Orellano 1, Néstor Carrillo 1, Nora B Calcaterra 1
PMCID: PMC1080220  PMID: 16668671

Abstract

Southern blot hybridization techniques were used to estimate the extent of chloroplast DNA sequences present in the mitochondrial genome of cowpea (Vigna unguiculata L.) The entire mitochondrial chromosome was homogeneously labeled and used to probe blotted DNA fragments obtained by extensive restriction of the tobacco chloroplast genome. The strongest cross-homologies were obtained with fragments derived from the inverted repeat and the atpBE cluster regions, although most of the clones tested (spanning 85% of the tobacco plastid genome) hybridized to mitochondrial DNA. Homologous chloroplast DNA restriction fragments represent a total of 30 to 68 kilobase pairs, depending upon the presence or absence of tRNA-encoding fragments. Plastid genes showing homology with mitochondrial DNA include those encoding ribosomal proteins, RNA polymerase, subunits of photosynthetic complexes, and the two major rRNAs.

Full text

PDF
525

Images in this article

526Figure 1
on p.526
527Figure 2
on p.527

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowman C. M., Dyer T. A. Purification and analysis of DNA from wheat chloroplasts isolated in nonaqueous media. Anal Biochem. 1982 May 1;122(1):108–118. doi: 10.1016/0003-2697(82)90258-5. [DOI] [PubMed] [Google Scholar]
  2. Dewey R. E., Levings C. S., 3rd, Timothy D. H. Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell. 1986 Feb 14;44(3):439–449. doi: 10.1016/0092-8674(86)90465-4. [DOI] [PubMed] [Google Scholar]
  3. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  4. Fejes E., Masters B. S., McCarty D. M., Hauswirth W. W. Sequence and transcriptional analysis of a chloroplast insert in the mitochondrial genome of Zea mays. Curr Genet. 1988 Jun;13(6):509–515. doi: 10.1007/BF02427757. [DOI] [PubMed] [Google Scholar]
  5. Lonsdale D. M., Hodge T. P., Howe C. J., Stern D. B. Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA. Cell. 1983 Oct;34(3):1007–1014. doi: 10.1016/0092-8674(83)90558-5. [DOI] [PubMed] [Google Scholar]
  6. Marano M. R., Carrillo N. Chromoplast formation during tomato fruit ripening. No evidence for plastid DNA methylation. Plant Mol Biol. 1991 Jan;16(1):11–19. doi: 10.1007/BF00017913. [DOI] [PubMed] [Google Scholar]
  7. Marchfelder A., Schuster W., Brennicke A. In vitro processing of mitochondrial and plastid derived tRNA precursors in a plant mitochondrial extract. Nucleic Acids Res. 1990 Mar 25;18(6):1401–1406. doi: 10.1093/nar/18.6.1401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Marechal L., Runeberg-Roos P., Grienenberger J. M., Colin J., Weil J. H., Lejeune B., Quetier F., Lonsdale D. M. Homology in the region containing a tRNA(Trp) gene and a (complete or partial) tRNA(Pro) gene in wheat mitochondrial and chloroplast genomes. Curr Genet. 1987;12(2):91–98. doi: 10.1007/BF00434662. [DOI] [PubMed] [Google Scholar]
  9. Moon E., Kao T. H., Wu R. Rice chloroplast DNA molecules are heterogeneous as revealed by DNA sequences of a cluster of genes. Nucleic Acids Res. 1987 Jan 26;15(2):611–630. doi: 10.1093/nar/15.2.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moon E., Kao T. H., Wu R. Rice mitochondrial genome contains a rearranged chloroplast gene cluster. Mol Gen Genet. 1988 Aug;213(2-3):247–253. doi: 10.1007/BF00339588. [DOI] [PubMed] [Google Scholar]
  11. Nugent J. M., Palmer J. D. Location, identity, amount and serial entry of chloroplast DNA sequences in crucifer mitochondrial DNAs. Curr Genet. 1988 Nov;14(5):501–509. doi: 10.1007/BF00521276. [DOI] [PubMed] [Google Scholar]
  12. Palmer J. D. Comparative organization of chloroplast genomes. Annu Rev Genet. 1985;19:325–354. doi: 10.1146/annurev.ge.19.120185.001545. [DOI] [PubMed] [Google Scholar]
  13. Reed K. C., Mann D. A. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 1985 Oct 25;13(20):7207–7221. doi: 10.1093/nar/13.20.7207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shinozaki K., Ohme M., Tanaka M., Wakasugi T., Hayashida N., Matsubayashi T., Zaita N., Chunwongse J., Obokata J., Yamaguchi-Shinozaki K. The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J. 1986 Sep;5(9):2043–2049. doi: 10.1002/j.1460-2075.1986.tb04464.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stern D. B. DNA transposition between plant organellar genomes. J Cell Sci Suppl. 1987;7:145–154. doi: 10.1242/jcs.1987.supplement_7.11. [DOI] [PubMed] [Google Scholar]
  16. Stern D. B., Lonsdale D. M. Mitochondrial and chloroplast genomes of maize have a 12-kilobase DNA sequence in common. Nature. 1982 Oct 21;299(5885):698–702. doi: 10.1038/299698a0. [DOI] [PubMed] [Google Scholar]
  17. Stern D. B., Newton K. J. Isolation of plant mitochondrial RNA. Methods Enzymol. 1986;118:488–496. doi: 10.1016/0076-6879(86)18095-5. [DOI] [PubMed] [Google Scholar]
  18. Stern D. B., Palmer J. D. Extensive and widespread homologies between mitochondrial DNA and chloroplast DNA in plants. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1946–1950. doi: 10.1073/pnas.81.7.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stern D. B., Palmer J. D. Tripartite mitochondrial genome of spinach: physical structure, mitochondrial gene mapping, and locations of transposed chloroplast DNA sequences. Nucleic Acids Res. 1986 Jul 25;14(14):5651–5666. doi: 10.1093/nar/14.14.5651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sugiura M. The chloroplast chromosomes in land plants. Annu Rev Cell Biol. 1989;5:51–70. doi: 10.1146/annurev.cb.05.110189.000411. [DOI] [PubMed] [Google Scholar]
  21. Wintz H., Grienenberger J. M., Weil J. H., Lonsdale D. M. Location and nucleotide sequence of two tRNA genes and a tRNA pseudo-gene in the maize mitochondrial genome: evidence for the transcription of a chloroplast gene in mitochondria. Curr Genet. 1988 Mar;13(3):247–254. doi: 10.1007/BF00387771. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES