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. 1992 Mar;98(3):1202–1206. doi: 10.1104/pp.98.3.1202

Plasma Membrane Ca-ATPase of Radish Seedlings 1

II. Regulation by Calmodulin

Franca Rasi-Caldogno 1,2, Antonella Carnelli 1,2, Maria I De Michelis 1,2
PMCID: PMC1080328  PMID: 16668747

Abstract

The effect of calmodulin on the activity of the plasma membrane Ca-ATPase was investigated on plasma membranes purified from radish (Raphanus sativus L.) seedlings. Calmodulin stimulated the hydrolytic activity and the transport activity of the plasma membrane Ca-ATPase to comparable extents in a manner dependent on the free Ca2+ concentration. Stimulation was marked at low, nonsaturating Ca2+ concentrations and decreased increasing Ca2+, so that the effect of calmodulin resulted in an increase of the apparent affinity of the enzyme for free Ca2+. The pattern of calmodulin stimulation of the plasma membrane Ca-ATPase activity was substantially the same at pH 6.9 and 7.5, in the presence of ATP or ITP, and when calmodulin from radish seeds was used rather than that from bovine brain. At pH 6.9 in the presence of 5 micromolar free Ca2+, stimulation of the plasma membrane Ca-ATPase was saturated by 30 to 50 micrograms per milliliter bovine brain calmodulin. The calmodulin antagonist calmidazolium inhibited both basal and calmodulin-stimulated plasma membrane Ca-ATPase activity to comparable extents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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