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. 1992 Apr;98(4):1498–1501. doi: 10.1104/pp.98.4.1498

Evidence of Two Isomers of Phosphatidylinositol in Plant Tissue 1

Pushpalatha P N Murthy 1,2, Gay Pliska-Matyshak 1,2, Lisa M Keranen 1,2, Philippe Lam 1,2, Hans H Mueller 1,2, N Bhuvarahamurthy 1,2
PMCID: PMC1080377  PMID: 16668820

Abstract

The structure of phosphatidylinositol in barley (Hordeum vulgare) aleurone layers was investigated by chemical degradation. In vivo myo-[2-3H]inositol-labeled phosphatidylinositol was first converted to glycerophosphoinositol and, subsequently, after removal of the glycerol moiety, to inositol monophosphate. Here, we present data that show that, in addition to the commonly occurring 1,2-diacylglycero-3-(d-myo-inositol-1-phosphate), barley aleurone cells contain a novel second isomer of phosphatidylinositol that differs in structure of the head group.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agranoff B. W., Murthy P., Seguin E. B. Thrombin-induced phosphodiesteratic cleavage of phosphatidylinositol bisphosphate in human platelets. J Biol Chem. 1983 Feb 25;258(4):2076–2078. [PubMed] [Google Scholar]
  2. Berridge M. J. Inositol trisphosphate and diacylglycerol: two interacting second messengers. Annu Rev Biochem. 1987;56:159–193. doi: 10.1146/annurev.bi.56.070187.001111. [DOI] [PubMed] [Google Scholar]
  3. Brown D. M., Stewart J. C. The structure of triphosphoinositide from beef brain. Biochim Biophys Acta. 1966 Dec 7;125(3):413–421. doi: 10.1016/0005-2760(66)90029-4. [DOI] [PubMed] [Google Scholar]
  4. Clarke N. G., Dawson R. M. Alkaline O leads to N-transacylation. A new method for the quantitative deacylation of phospholipids. Biochem J. 1981 Apr 1;195(1):301–306. doi: 10.1042/bj1950301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coté G. G., Depass A. L., Quarmby L. M., Tate B. F., Morse M. J., Satter R. L., Crain R. C. Separation and Characterization of Inositol Phospholipids from the Pulvini of Samanea saman. Plant Physiol. 1989 Aug;90(4):1422–1428. doi: 10.1104/pp.90.4.1422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cunha-Melo J. R., Dean N. M., Ali H., Beaven M. A. Formation of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate from inositol 1,3,4,5-tetrakisphosphate and their pathways of degradation in RBL-2H3 cells. J Biol Chem. 1988 Oct 5;263(28):14245–14250. [PubMed] [Google Scholar]
  7. Dean N. M., Moyer J. D. Separation of multiple isomers of inositol phosphates formed in GH3 cells. Biochem J. 1987 Mar 1;242(2):361–366. doi: 10.1042/bj2420361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ferguson M. A., Williams A. F. Cell-surface anchoring of proteins via glycosyl-phosphatidylinositol structures. Annu Rev Biochem. 1988;57:285–320. doi: 10.1146/annurev.bi.57.070188.001441. [DOI] [PubMed] [Google Scholar]
  9. Futerman A. H., Low M. G., Ackermann K. E., Sherman W. R., Silman I. Identification of covalently bound inositol in the hydrophobic membrane-anchoring domain of Torpedo acetylcholinesterase. Biochem Biophys Res Commun. 1985 May 31;129(1):312–317. doi: 10.1016/0006-291x(85)91439-1. [DOI] [PubMed] [Google Scholar]
  10. Irvine R. F., Letcher A. J., Lander D. J., Drøbak B. K., Dawson A. P., Musgrave A. Phosphatidylinositol(4,5)bisphosphate and Phosphatidylinositol(4)phosphate in Plant Tissues. Plant Physiol. 1989 Mar;89(3):888–892. doi: 10.1104/pp.89.3.888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Irvine R. Cell physiology. Messenger gets the green light. Nature. 1990 Aug 23;346(6286):700–701. doi: 10.1038/346700a0. [DOI] [PubMed] [Google Scholar]
  12. Mato J. M., Kelly K. L., Abler A., Jarett L., Corkey B. E., Cashel J. A., Zopf D. Partial structure of an insulin-sensitive glycophospholipid. Biochem Biophys Res Commun. 1987 Jul 31;146(2):764–770. doi: 10.1016/0006-291x(87)90595-x. [DOI] [PubMed] [Google Scholar]
  13. Murthy P. P. A fast and easy technique for the isolation of aleurone layers. Plant Physiol. 1989 Jun;90(2):388–389. doi: 10.1104/pp.90.2.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murthy P. P., Renders J. M., Keranen L. M. Phosphoinositides in barley aleurone layers and gibberellic Acid-induced changes in metabolism. Plant Physiol. 1989 Dec;91(4):1266–1269. doi: 10.1104/pp.91.4.1266. [DOI] [PMC free article] [PubMed] [Google Scholar]

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