Abstract
Mitochondrial and plastid gene expression has been examined during maize (Zea mays) microsporogenesis. Accumulation of transcripts was found for three mitochondrial genes studied (cob, atp6, and atp9) at the mid-term of pollen development. In contrast, these mitochondrial transcripts were undetectable in mature pollen. Southern and DNA gel blot experiments showed that the copy number of mitochondrial genes was amplified in microspores at stages preceding the accumulation of these transcripts. Plastid transcripts of the photosynthetic psbA and rbcL genes could not be detected after the two mitoses, whereas precursors of the 16S rRNA are detected at low levels.
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- Briat J. F., Dron M., Loiseaux S., Mache R. Structure and transcription of the spinach chloroplast rDNA leader region. Nucleic Acids Res. 1982 Nov 11;10(21):6865–6878. doi: 10.1093/nar/10.21.6865. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dawson A. J., Jones V. P., Leaver C. J. The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site. EMBO J. 1984 Sep;3(9):2107–2113. doi: 10.1002/j.1460-2075.1984.tb02098.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Day A., Ellis T. H. Chloroplast DNA deletions associated with wheat plants regenerated from pollen: possible basis for maternal inheritance of chloroplasts. Cell. 1984 Dec;39(2 Pt 1):359–368. doi: 10.1016/0092-8674(84)90014-x. [DOI] [PubMed] [Google Scholar]
- Dewey R. E., Levings C. S., 3rd, Timothy D. H. Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell. 1986 Feb 14;44(3):439–449. doi: 10.1016/0092-8674(86)90465-4. [DOI] [PubMed] [Google Scholar]
- Dewey R. E., Levings C. S., Timothy D. H. Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol. 1985 Nov;79(3):914–919. doi: 10.1104/pp.79.3.914. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dewey R. E., Schuster A. M., Levings C. S., Timothy D. H. Nucleotide sequence of F(0)-ATPase proteolipid (subunit 9) gene of maize mitochondria. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1015–1019. doi: 10.1073/pnas.82.4.1015. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fauron C. M., Havlik M. The BamHI, XhoI, SmaI restriction enzyme maps of the normal maize mitochondrial genome genotype B37. Nucleic Acids Res. 1988 Nov 11;16(21):10395–10396. doi: 10.1093/nar/16.21.10395. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kobayashi H., Ngernprasirtsiri J., Akazawa T. Transcriptional regulation and DNA methylation in plastids during transitional conversion of chloroplasts to chromoplasts. EMBO J. 1990 Feb;9(2):307–313. doi: 10.1002/j.1460-2075.1990.tb08112.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lonsdale D. M., Hodge T. P., Howe C. J., Stern D. B. Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA. Cell. 1983 Oct;34(3):1007–1014. doi: 10.1016/0092-8674(83)90558-5. [DOI] [PubMed] [Google Scholar]
- Macherel D., Kobayashi H., Akazawa T., Kawano S., Kuroiwa T. Amyloplast nucleoids in sycamore cells and presence in amyloplast DNA of homologous sequences to chloroplast genes. Biochem Biophys Res Commun. 1985 Nov 27;133(1):140–146. doi: 10.1016/0006-291x(85)91852-2. [DOI] [PubMed] [Google Scholar]
- Rottmann W. H., Brears T., Hodge T. P., Lonsdale D. M. A mitochondrial gene is lost via homologous recombination during reversion of CMS T maize to fertility. EMBO J. 1987 Jun;6(6):1541–1546. doi: 10.1002/j.1460-2075.1987.tb02398.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sederoff R. R., Ronald P., Bedinger P., Rivin C., Walbot V., Bland M., Levings C. S., 3rd Maize mitochondrial plasmid S-1 sequences share homology with chloroplast gene psbA. Genetics. 1986 Jun;113(2):469–482. doi: 10.1093/genetics/113.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stern D. B., Lonsdale D. M. Mitochondrial and chloroplast genomes of maize have a 12-kilobase DNA sequence in common. Nature. 1982 Oct 21;299(5885):698–702. doi: 10.1038/299698a0. [DOI] [PubMed] [Google Scholar]
- Stern D. B., Palmer J. D. Extensive and widespread homologies between mitochondrial DNA and chloroplast DNA in plants. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1946–1950. doi: 10.1073/pnas.81.7.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas F., Massenet O., Dorne A. M., Briat J. F., Mache R. Expression of the rpl23, rpl2 and rps19 genes in spinach chloroplasts. Nucleic Acids Res. 1988 Mar 25;16(6):2461–2472. doi: 10.1093/nar/16.6.2461. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Warmke H. E., Lee S. L. Pollen Abortion in T Cytoplasmic Male-Sterile Corn (Zea mays): A Suggested Mechanism. Science. 1978 May 5;200(4341):561–563. doi: 10.1126/science.200.4341.561. [DOI] [PubMed] [Google Scholar]
- Young E. G., Hanson M. R. A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell. 1987 Jul 3;50(1):41–49. doi: 10.1016/0092-8674(87)90660-x. [DOI] [PubMed] [Google Scholar]
- Zurawski G., Perrot B., Bottomley W., Whitfeld P. R. The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA. Nucleic Acids Res. 1981 Jul 24;9(14):3251–3270. doi: 10.1093/nar/9.14.3251. [DOI] [PMC free article] [PubMed] [Google Scholar]