Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1992 Aug;99(4):1699–1703. doi: 10.1104/pp.99.4.1699

Differential Accumulation of Biotin Enzymes during Carrot Somatic Embryogenesis 1

Eve Syrkin Wurtele 1,2, Basil J Nikolau 1,2
PMCID: PMC1080686  PMID: 16669096

Abstract

The activities of four biotin enzymes, acetyl-coenzyme A (CoA) carboxylase, 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase, and the accumulation of six biotin-containing polypeptides were determined during development of somatic embryos of carrot (Daucus carota). Acetyl-CoA carboxylase activity increased more than sevenfold, whereas the activities of 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase were relatively unaltered. An increase also occurred in the accumulation of three of the biotin-containing polypeptides (molecular masses of 220, 62, and 34 kilodaltons). Of these, the most dramatic change was in the accumulation of the 62-kilodalton biotin-containing polypeptide, which increased by at least 50-fold as embryogenic cell clusters developed into torpedo embryos.

Full text

PDF
1699

Images in this article

1701Figure 1
on p.1701

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Egin-Bühler B., Ebel J. Improved purification and further characterization of acetyl-CoA carboxylase from cultured cells of parsley (Petroselinum hortense). Eur J Biochem. 1983 Jun 15;133(2):335–339. doi: 10.1111/j.1432-1033.1983.tb07467.x. [DOI] [PubMed] [Google Scholar]
  2. Egin-Bühler B., Loyal R., Ebel J. Comparison of acetyl-CoA carboxylases from parsley cell cultures and wheat germ. Arch Biochem Biophys. 1980 Aug;203(1):90–100. doi: 10.1016/0003-9861(80)90156-3. [DOI] [PubMed] [Google Scholar]
  3. Fujimura T., Komamine A. Synchronization of somatic embryogenesis in a carrot cell suspension culture. Plant Physiol. 1979 Jul;64(1):162–164. doi: 10.1104/pp.64.1.162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Keller G. L., Nikolau B. J., Ulrich T. H., Wurtele E. S. Comparison of Starch and ADP-Glucose Pyrophosphorylase Levels in Nonembryogenic Cells and Developing Embryos from Induced Carrot Cultures. Plant Physiol. 1988 Feb;86(2):451–456. doi: 10.1104/pp.86.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lane M. D., Moss J., Polakis S. E. Acetyl coenzyme A carboxylase. Curr Top Cell Regul. 1974;8(0):139–195. [PubMed] [Google Scholar]
  6. Nikolau B. J., Hawke J. C. Purification and characterization of maize leaf acetyl-coenzyme A carboxylase. Arch Biochem Biophys. 1984 Jan;228(1):86–96. doi: 10.1016/0003-9861(84)90049-3. [DOI] [PubMed] [Google Scholar]
  7. Nikolau B. J., Wurtele E. S., Stumpf P. K. Tissue distribution of acetyl-coenzyme a carboxylase in leaves. Plant Physiol. 1984 Aug;75(4):895–901. doi: 10.1104/pp.75.4.895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nikolau B. J., Wurtele E. S., Stumpf P. K. Use of streptavidin to detect biotin-containing proteins in plants. Anal Biochem. 1985 Sep;149(2):448–453. doi: 10.1016/0003-2697(85)90596-2. [DOI] [PubMed] [Google Scholar]
  9. Turnham E., Northcote D. H. Changes in the activity of acetyl-CoA carboxylase during rape-seed formation. Biochem J. 1983 Apr 15;212(1):223–229. doi: 10.1042/bj2120223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wood H. G., Barden R. E. Biotin enzymes. Annu Rev Biochem. 1977;46:385–413. doi: 10.1146/annurev.bi.46.070177.002125. [DOI] [PubMed] [Google Scholar]
  11. Wurtele E. S., Nikolau B. J. Plants contain multiple biotin enzymes: discovery of 3-methylcrotonyl-CoA carboxylase, propionyl-CoA carboxylase and pyruvate carboxylase in the plant kingdom. Arch Biochem Biophys. 1990 Apr;278(1):179–186. doi: 10.1016/0003-9861(90)90246-u. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES