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. 1991 May;96(1):239–244. doi: 10.1104/pp.96.1.239

Ethylene Inhibitors Restore Nodulation to sym 5 Mutants of Pisum sativum L. cv Sparkle 1,2

Jeffrey C Fearn 1, Thomas A LaRue 1
PMCID: PMC1080739  PMID: 16668158

Abstract

The sym 5 mutants of pea, Pisum sativum L. cv Sparkle, do not differ in growth habit from their normal parent and nodulate poorly at a root temperature of 20°C. If inhibitors of ethylene formation or action (Co2+, aminoethoxyvinylglycine, or Ag+) are added to the substrate, nodulation of the sym 5 mutants is increased. Similar treatments of four other mutant sym lines do not restore nodulation. When Ag+ is added to the substrate from 4 days before to 4 days after inoculation with rhizobia, nodulation of sym 5 mutants is increased. The roots of the mutant need only be exposed to Ag+ for 4 hours to significantly increase nodule numbers. The content of free 1-aminocyclopropane-1-carboxylic acid and the production of ethylene in the lateral roots of sym 5 mutants do not differ from Sparkle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beyer E. M. A potent inhibitor of ethylene action in plants. Plant Physiol. 1976 Sep;58(3):268–271. doi: 10.1104/pp.58.3.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bleecker A. B., Estelle M. A., Somerville C., Kende H. Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana. Science. 1988 Aug 26;241(4869):1086–1089. doi: 10.1126/science.241.4869.1086. [DOI] [PubMed] [Google Scholar]
  3. Fearn J. C., LaRue T. A. An altered constitutive peptide in sym 5 mutants of Pisum sativum L. Plant Mol Biol. 1990 Feb;14(2):207–216. doi: 10.1007/BF00018561. [DOI] [PubMed] [Google Scholar]
  4. Fujino D. W., Burger D. W., Yang S. F., Bradford K. J. Characterization of an Ethylene Overproducing Mutant of Tomato (Lycopersicon esculentum Mill. Cultivar VFN8). Plant Physiol. 1988 Nov;88(3):774–779. doi: 10.1104/pp.88.3.774. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guzmán P., Ecker J. R. Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. Plant Cell. 1990 Jun;2(6):513–523. doi: 10.1105/tpc.2.6.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Peters N. K., Crist-Estes D. K. Nodule formation is stimulated by the ethylene inhibitor aminoethoxyvinylglycine. Plant Physiol. 1989 Oct;91(2):690–693. doi: 10.1104/pp.91.2.690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Van Brussel A. A., Zaat S. A., Cremers H. C., Wijffelman C. A., Pees E., Tak T., Lugtenberg B. J. Role of plant root exudate and Sym plasmid-localized nodulation genes in the synthesis by Rhizobium leguminosarum of Tsr factor, which causes thick and short roots on common vetch. J Bacteriol. 1986 Feb;165(2):517–522. doi: 10.1128/jb.165.2.517-522.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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