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. 1991 Jun;96(2):390–397. doi: 10.1104/pp.96.2.390

Differential Regulation of β-1,3-Glucanase Messenger RNAs in Response to Pathogen Infection

Eric R Ward 1,2,3, George B Payne 1,2,3, Mary B Moyer 1,2,3, Shericca C Williams 1,2,3, Sandra S Dincher 1,2,3, Kevin C Sharkey 1,2,3, James J Beck 1,2,3, Hope T Taylor 1,2,3, Patricia Ahl-Goy 1,2,3, Frederick Meins Jr 1,2,3, John A Ryals 1,2,3
PMCID: PMC1080782  PMID: 16668198

Abstract

The acidic, extracellular, glucan endo-1,3-β-glucosidases (EC 3.2.1.39; β-1,3-glucanases), pathogenesis-related proteins-2, -N, and -O (i.e. PR-2, PR-N, and PR-O) were purified from Nicotiana tabacum (tobacco) and their partial amino acid sequences determined. Based on these data, complementary DNA (cDNA) clones encoding the proteins were isolated. Additional cDNAs were isolated that encoded proteins approximately 90% identical with PR-2, PR-N, and PR-O. Although the proteins encoded by these cDNAs have not been identified, their deduced amino acid sequences have slightly basic or neutral calculated isoelectric points, as well as carboxy-terminal extensions. These physical characteristics are shared by the vacuolar form of β-1,3-glucanase and other vacuolar localized analogs of PR proteins, suggesting that the unidentified proteins may be similarly localized. A preliminary evolutionary model that separates the β-1,3-glucanase gene family from tobacco into at least five distinct subfamilies is proposed. The expression of β-1,3-glucanase messenger RNAs (mRNAs) in response to infection by tobacco mosaic virus was examined. Messages for the acidic glucanases were induced similarly to the mRNAs for other PR proteins. However, the basic glucanase showed a different response, suggesting that different isoforms are differentially regulated by tobacco mosaic virus infection at the mRNA level.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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