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. 1991 Jun;96(2):571–576. doi: 10.1104/pp.96.2.571

Ethylene Biosynthesis-Inducing Xylanase 1

III. Product Characterization

Jeffrey F D Dean 1,2,2, Kenneth C Gross 1,2, James D Anderson 1,2
PMCID: PMC1080808  PMID: 16668223

Abstract

Induction of ethylene biosynthesis in tobacco (Nicotiana tabacum cv Xanthi) leaf discs by the ethylene biosynthesis-inducing xylanase (EIX) isolated from Cellulysin or xylan-grown cultures of Trichoderma viride was dependent upon the concentration of xylanase applied and upon the length of incubation. Arrhenius activation energies of 9,100 and 10,500 calories for the Cellulysin and T. viride EIX xylanase activities, respectively, were derived from the Km and Vmax values determined for each enzyme at several temperatures. The two xylanases digested xylan in a strictly endo fashion, releasing neither xylobiose nor free xylose, and no debranching activity was associated with either enzyme. The xylanases released polysaccharides from ground corn cobs, but little or no carbohydrate was released from tobacco mesophyll cell walls incubated with EIX. No heat-stable products capable of inducing ethylene biosynthesis in tobacco leaf discs were found in EIX digests of purified xylans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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