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. 1991 Jul;96(3):768–774. doi: 10.1104/pp.96.3.768

Wheat Inhibitors of Heterologous α-Amylases 1

Characterization of Major Components from the Monomeric Class

Luis Gomez 1,2, Rosa Sanchez-Monge 1,2, Carlos Lopez-Otín 1,2, Gabriel Salcedo 1,2
PMCID: PMC1080842  PMID: 16668253

Abstract

The four major components of the wheat monomeric α-amylase inhibitors (WMAI) from wheat, Triticum aestivum, endosperm have been isolated and characterized. Two of them, WMAI-1 and WMAI-2, are highly active against the α-amylase from the insect Tenebrio molitor and their N-terminal amino acid sequences indicate that they are closely related to each other (86% identical residues) and to the other members of the family (subunits of dimeric and tetrameric α-amylase inhibitors and trypsin inhibitors). WMAI-1, which is identical to the previously described 0.28 inhibitor, is encoded by a gene located in the short arm of chromosome 6D and WMAI-2 by a gene in the short arm of chromosome 6B. Components 3 and 4, which have blocked N-terminal residues, have identical internal amino acid sequences and are a separate class of proteins with respect to WMAI-1 and WMAI-2, although their amino acid composition and apparent molecular weights are quite similar. Their inhibitory activity versus α-amylases is either unstable during the purification process or due to contamination with other inhibitors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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