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. 1991 Jul;96(3):892–897. doi: 10.1104/pp.96.3.892

Betaines of Alfalfa 1

Characterization by Fast Atom Bombardment and Desorption Chemical Ionization Mass Spectrometry

Karl V Wood 1,2,3,4,5, Kelly J Stringham 1,2,3,4,5, David L Smith 1,2,3,4,5, Jeffrey J Volenec 1,2,3,4,5, Kerry L Hendershot 1,2,3,4,5, Kimberly A Jackson 1,2,3,4,5, Patrick J Rich 1,2,3,4,5, Wen-Ju Yang 1,2,3,4,5, David Rhodes 1,2,3,4,5
PMCID: PMC1080861  PMID: 16668271

Abstract

Leaf tissue of alfalfa (Medicago sativa L.) was found to contain prolinebetaine, pipecolatebetaine, hydroxyprolinebetaine, and glycinebetaine. As n-butyl esters, these chemical species exhibit molecular cations at mass/charge ratio (m/z) 200, 214, 216, and 174, respectively, when analyzed by fast atom bombardment mass spectrometry. The underivatized betaines exhibit protonated molecular ions at m/z 144, 158, 160, and 118, respectively, when analyzed by desorption chemical ionization mass spectrometry. Extensive (>45-fold) genotypic variation for hydroxyprolinebetaine level was identified in alfalfa. Because a significant inverse correlation between prolinebetaine and hydroxyprolinebetaine levels was observed among 15 alfalfa genotypes evaluated, it is possible that these compounds may be derived from a common intermediate. Birdsfoot trefoil (Lotus corniculatus L.) contained prolinebetaine, but only traces of glycinebetaine, pipecolatebetaine, and hydroxyprolinebetaine. Red clover (Trifolium pratense L.) lacked prolinebetaine, pipecolatebetaine, and hydroxyprolinebetaine, but contained appreciable levels of both glycinebetaine and trigonelline. Trigonelline was not detectable in the leaf tissue of any alfalfa genotype or cultivar evaluated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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