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. 1991 Jul;96(3):922–927. doi: 10.1104/pp.96.3.922

Light Regulation of Sink Metabolism in Tomato Fruit 1

II. Carbohydrate Metabolizing Enzymes

Han Ping Guan 1, Harry W Janes 1
PMCID: PMC1080866  PMID: 16668276

Abstract

Effects of light on carbohydrate levels and certain carbon metabolizing enzyme activities were studied during the early development of tomato (Lycopersicon esculentum) fruit. Sucrose levels were low and continued to decline during development and were unaffected by light. Starch was significantly greater in light. Invertase activity was similar in both light- and dark-grown fruit. Sucrose synthase activity was much lower than invertase and showed a slight decrease in light-grown fruit between days 21 and 28. Light-grown fruit also had higher ADP glucose pyrophosphorylase activity than dark-grown fruit, which was correlated with higher starch levels. The rapidly decreasing activity of ADP glucose pyrophosphorylase during early fruit development in the dark in conjunction with reduced starch levels and rates of accumulation indicates that ADP glucose pyrophosphorylase is crucial for carbon import and storage in tomato. The differential stimulation of ADP glucose pyrophosphorylase activity from light- and dark-grown tissue by 3-phosphoglycerate suggests that this enzyme may be allosterically altered by light.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chourey P. S., Nelson O. E. The enzymatic deficiency conditioned by the shrunken-1 mutations in maize. Biochem Genet. 1976 Dec;14(11-12):1041–1055. doi: 10.1007/BF00485135. [DOI] [PubMed] [Google Scholar]
  2. Damon S., Hewitt J., Nieder M., Bennett A. B. Sink Metabolism in Tomato Fruit : II. Phloem Unloading and Sugar Uptake. Plant Physiol. 1988 Jul;87(3):731–736. doi: 10.1104/pp.87.3.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Guan H. P., Janes H. W. Light regulation of sink metabolism in tomato fruit : I. Growth and sugar accumulation. Plant Physiol. 1991 Jul;96(3):916–921. doi: 10.1104/pp.96.3.916. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Keller G. L., Nikolau B. J., Ulrich T. H., Wurtele E. S. Comparison of Starch and ADP-Glucose Pyrophosphorylase Levels in Nonembryogenic Cells and Developing Embryos from Induced Carrot Cultures. Plant Physiol. 1988 Feb;86(2):451–456. doi: 10.1104/pp.86.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lingle S. E., Dunlap J. R. Sucrose Metabolism in Netted Muskmelon Fruit during Development. Plant Physiol. 1987 Jun;84(2):386–389. doi: 10.1104/pp.84.2.386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Murata T., Sugiyama T., Minamikawa T., Akazawa T. Enzymic mechanism of starch synthesis in ripening rice grains. 3. Mechanism of the sucrose-starch conversion. Arch Biochem Biophys. 1966 Jan;113(1):34–44. doi: 10.1016/0003-9861(66)90153-6. [DOI] [PubMed] [Google Scholar]
  7. Plaxton W. C., Preiss J. Purification and Properties of Nonproteolytic Degraded ADPglucose Pyrophosphorylase from Maize Endosperm. Plant Physiol. 1987 Jan;83(1):105–112. doi: 10.1104/pp.83.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Robinson N. L., Hewitt J. D., Bennett A. B. Sink metabolism in tomato fruit : I. Developmental changes in carbohydrate metabolizing enzymes. Plant Physiol. 1988 Jul;87(3):727–730. doi: 10.1104/pp.87.3.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]
  10. Sowokinos J. R. Pyrophosphorylases in Solanum tuberosum: I. Changes in ADP-Glucose and UDP-Glucose Pyrophosphorylase Activities Associated with Starch Biosynthesis during Tuberization, Maturation, and Storage of Potatoes. Plant Physiol. 1976 Jan;57(1):63–68. doi: 10.1104/pp.57.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Spilatro S. R., Preiss J. Regulation of Starch Synthesis in the Bundle Sheath and Mesophyll of Zea mays L. : Intercellular Compartmentalization of Enzymes of Starch Metabolism and the Properties of the ADPglucose Pyrophosphorylases. Plant Physiol. 1987 Mar;83(3):621–627. doi: 10.1104/pp.83.3.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wolosiuk R. A., Pontis H. G. The role of sucrose and sucrose synthetase in carbohydrate plant metabolism. Mol Cell Biochem. 1974 Sep 30;4(2):115–123. doi: 10.1007/BF01770292. [DOI] [PubMed] [Google Scholar]

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