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. 1991 Aug;96(4):1014–1017. doi: 10.1104/pp.96.4.1014

Sucrose Phosphate Is Not Transported into Vacuoles or Tonoplast Vesicles from Red Beet (Beta vulgaris) Hypocotyl 1

Ed Echeverria 1,2, Michael E Salvucci 1,2
PMCID: PMC1080886  PMID: 16668291

Abstract

Tonoplast vesicles and vacuoles isolated from red beet (Beta vulgaris L.) hypocotyl accumulated externally supplied [14C]sucrose but not [14C]sucrose phosphate despite the occurrence of sucrose phosphate phosphohydrolytic activity in the vacuole. The activities of sucrose synthase and sucrose phosphate synthase in whole cell extracts were 960 and 30 nanomoles per milligram protein per minute, respectively; whereas, no sucrose synthesizing activity was measured in tonoplast preparations. The results obtained in this investigation are incompatible with the involvement of sucrose phosphate synthase in the process of sucrose synthesis and accumulation in the storage cells of red beet.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIRD I. F., PORTER H. K., STOCKING C. R. INTRACELLULAR LOCALISATION OF ENZYMES ASSOCIATED WITH SUCROSE SYNTHESIS IN LEAVES. Biochim Biophys Acta. 1965 May 4;100:366–375. doi: 10.1016/0304-4165(65)90005-x. [DOI] [PubMed] [Google Scholar]
  2. Briskin D. P., Thornley W. R., Wyse R. E. Membrane Transport in Isolated Vesicles from Sugarbeet Taproot : II. Evidence for a Sucrose/H-Antiport. Plant Physiol. 1985 Aug;78(4):871–875. doi: 10.1104/pp.78.4.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Giaquinta R. T. Sucrose translocation and storage in the sugar beet. Plant Physiol. 1979 May;63(5):828–832. doi: 10.1104/pp.63.5.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hawker J. S., Smith G. M., Phillips H., Wiskich J. T. Sucrose phosphatase associated with vacuole preparations from red beet, sugar beet, and immature sugarcane stem. Plant Physiol. 1987 Aug;84(4):1281–1285. doi: 10.1104/pp.84.4.1281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hubbard N. L., Huber S. C., Pharr D. M. Sucrose Phosphate Synthase and Acid Invertase as Determinants of Sucrose Concentration in Developing Muskmelon (Cucumis melo L.) Fruits. Plant Physiol. 1989 Dec;91(4):1527–1534. doi: 10.1104/pp.91.4.1527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hubbard N. L., Pharr D. M., Huber S. C. Role of sucrose phosphate synthase in sucrose biosynthesis in ripening bananas and its relationship to the respiratory climacteric. Plant Physiol. 1990 Sep;94(1):201–208. doi: 10.1104/pp.94.1.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lingle S. E. Evidence for the uptake of sucrose intact into sugarcane internodes. Plant Physiol. 1989 May;90(1):6–8. doi: 10.1104/pp.90.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Maretzki A., Thom M. A group translocator for sucrose assimilation in tonoplast vesicles of sugarcane cells. Plant Physiol. 1986 Jan;80(1):34–37. doi: 10.1104/pp.80.1.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Salvucci M. E., Drake R. R., Haley B. E. Purification and photoaffinity labeling of sucrose phosphate synthase from spinach leaves. Arch Biochem Biophys. 1990 Sep;281(2):212–218. doi: 10.1016/0003-9861(90)90434-z. [DOI] [PubMed] [Google Scholar]
  10. Smyth D. A., Prescott H. E. Sugar content and activity of sucrose metabolism enzymes in milled rice grain. Plant Physiol. 1989 Mar;89(3):893–896. doi: 10.1104/pp.89.3.893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Usuda H., Edwards G. E. Localization of glycerate kinase and some enzymes for sucrose synthesis in c(3) and c(4) plants. Plant Physiol. 1980 May;65(5):1017–1022. doi: 10.1104/pp.65.5.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wyse R. Sucrose uptake by sugar beet tap root tissue. Plant Physiol. 1979 Nov;64(5):837–841. doi: 10.1104/pp.64.5.837. [DOI] [PMC free article] [PubMed] [Google Scholar]

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