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. 1991 Aug;96(4):1048–1052. doi: 10.1104/pp.96.4.1048

Heat Shock Causes Selective Destabilization of Secretory Protein mRNAs in Barley Aleurone Cells 1

Mark R Brodl 1,2,2, Tuan-hua David Ho 1,2
PMCID: PMC1080891  PMID: 16668296

Abstract

The aleurone layer of GA3-stimulated barley (Hordeum vulgare L., cv Himalaya) grains is normally devoted to the synthesis and secretion of hydrolytic enzymes. Heat shock, however, suppresses the synthesis of the main hydrolytic enzyme, α-amylase, by destabilizing its otherwise highly stable mRNA (FC Belanger, MR Brodl, T-hD Ho [1986] Proc Natl Acad Sci USA 83: 1354-1358). In this paper we document that heat shock causes the suppression of the synthesis of some normal cellular proteins, while the synthesis of other normal cellular proteins is unaffected by heat shock. There are two major isozymic forms of α-amylase encoded by distinct mRNAs. The mRNA levels for both isozymic forms and the mRNA levels of two other secretory proteins, a protease and an endochitinase, were markedly reduced during heat shock. However, the levels of actin and β-tubulin mRNAs, both nonsecretory proteins, were not diminished during heat shock. In addition, the levels of three other mRNA species detected by a set of unidentified cDNA clones (the sequence of one shows that it lacks a signal sequence) remained unchanged during heat shock. These data indicate that there are two classes of normal cellular protein mRNAs with regard to the effect of heat shock upon their persistence in the cell, and suggest that the distinction between them is whether or not they encode secretory proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belanger F. C., Brodl M. R., Ho T. H. Heat shock causes destabilization of specific mRNAs and destruction of endoplasmic reticulum in barley aleurone cells. Proc Natl Acad Sci U S A. 1986 Mar;83(5):1354–1358. doi: 10.1073/pnas.83.5.1354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brock M. L., Shapiro D. J. Estrogen stabilizes vitellogenin mRNA against cytoplasmic degradation. Cell. 1983 Aug;34(1):207–214. doi: 10.1016/0092-8674(83)90151-4. [DOI] [PubMed] [Google Scholar]
  3. Callis J., Ho T. H. Multiple molecular forms of the gibberellin-induced alpha-amylase from the aleurone layers of barley seeds. Arch Biochem Biophys. 1983 Jul 1;224(1):224–234. doi: 10.1016/0003-9861(83)90206-0. [DOI] [PubMed] [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  5. Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DiDomenico B. J., Bugaisky G. E., Lindquist S. Heat shock and recovery are mediated by different translational mechanisms. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6181–6185. doi: 10.1073/pnas.79.20.6181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harper J. F., Mages W. Organization and structure of Volvox beta-tubulin genes. Mol Gen Genet. 1988 Aug;213(2-3):315–324. doi: 10.1007/BF00339597. [DOI] [PubMed] [Google Scholar]
  8. Koehler S. M., Ho T. H. Hormonal regulation, processing, and secretion of cysteine proteinases in barley aleurone layers. Plant Cell. 1990 Aug;2(8):769–783. doi: 10.1105/tpc.2.8.769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Lindquist S. Regulation of protein synthesis during heat shock. Nature. 1981 Sep 24;293(5830):311–314. doi: 10.1038/293311a0. [DOI] [PubMed] [Google Scholar]
  11. Muthukrishnan S., Chandra G. R., Maxwell E. S. Hormonal control of alpha-amylase gene expression in barley. Studies using a cloned CDNA probe. J Biol Chem. 1983 Feb 25;258(4):2370–2375. [PubMed] [Google Scholar]
  12. Shah D. M., Hightower R. C., Meagher R. B. Complete nucleotide sequence of a soybean actin gene. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1022–1026. doi: 10.1073/pnas.79.4.1022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Storti R. V., Scott M. P., Rich A., Pardue M. L. Translational control of protein synthesis in response to heat shock in D. melanogaster cells. Cell. 1980 Dec;22(3):825–834. doi: 10.1016/0092-8674(80)90559-0. [DOI] [PubMed] [Google Scholar]
  14. Vierling E., Key J. L. Ribulose 1,5-Bisphosphate Carboxylase Synthesis during Heat Shock. Plant Physiol. 1985 May;78(1):155–162. doi: 10.1104/pp.78.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wolffe A. P., Perlman A. J., Tata J. R. Transient paralysis by heat shock of hormonal regulation of gene expression. EMBO J. 1984 Dec 1;3(12):2763–2770. doi: 10.1002/j.1460-2075.1984.tb02207.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

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