Abstract
A mutant of Arabidopsis thaliana that is sensitive to exogenous l-arabinose has been isolated. Comparisons of growth of the wild type, mutant, and F1 and F2 progeny of crosses showed the arabinose-sensitive phenotype is semidominant and segregates as a single Mendelian locus. Crosses of the mutant to marker strains showed the mutation is linked to the eceriferum-2 locus on chromosome 4. In vivo incorporation of exogenous labeled l-arabinose into ethanol-insoluble polysaccharides was greatly reduced in the mutant with a concomitant accumulation of free labeled arabinose. Enzyme assays of crude plant extracts demonstrated a defect in arabinose kinase activity in the mutant.
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- Chan P. H., Hassid W. Z. One step purification of D-galactose and L-arabinose kinases from Phaseolus aureus seedlings by ATP-sepharose affinity chromatography. Anal Biochem. 1975 Apr;64(2):372–379. doi: 10.1016/0003-2697(75)90445-5. [DOI] [PubMed] [Google Scholar]
- Johnson K. D., Daniels D., Dowler M. J., Rayle D. L. Activation of Avena coleoptile cell wall glycosidases by hydrogen ions and auxin. Plant Physiol. 1974 Feb;53(2):224–228. doi: 10.1104/pp.53.2.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Masuda Y., Yamamoto R. Effect of auxin on beta-1, 3-glucanase activity in Avena coleoptile. Dev Growth Differ. 1970 Mar;11(4):287–296. doi: 10.1111/j.1440-169x.1970.00287.x. [DOI] [PubMed] [Google Scholar]
- NEUFELD E. F., GINSBURG V., PUTMAN E. W., FANSHIER D., HASSID W. Z. Formation and interconversion of sugar nucleotides by plant extracts. Arch Biochem Biophys. 1957 Jul;69:602–616. doi: 10.1016/0003-9861(57)90524-6. [DOI] [PubMed] [Google Scholar]
- Suiter K. A., Wendel J. F., Case J. S. LINKAGE-1: a PASCAL computer program for the detection and analysis of genetic linkage. J Hered. 1983 May-Jun;74(3):203–204. doi: 10.1093/oxfordjournals.jhered.a109766. [DOI] [PubMed] [Google Scholar]