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. 1991 Oct;97(2):644–650. doi: 10.1104/pp.97.2.644

Transport of Arginine and Aspartic Acid into Isolated Barley Mesophyll Vacuoles 1

Enrico Martinoia 1,2, Monika Thume 1,2, Esther Vogt 1,2, Doris Rentsch 1,2, Karl-Josef Dietz 1,2
PMCID: PMC1081055  PMID: 16668447

Abstract

The transport of arginine into isolated barley (Hordeum vulgare L.) mesophyll vacuoles was investigated. In the absence of ATP, arginine uptake was saturable with a Km of 0.3 to 0.4 millimolar. Positively charged amino acids inhibited arginine uptake, lysine being most potent with a Ki of 1.2 millimolar. In the presence of free ATP, but not of its Mg-complex, uptake of arginine was drastically enhanced and a linear function of its concentration up to 16 millimolar. The nonhydrolyzable adenylyl imidodiphosphate, but no other nucleotide tested, could substitute for ATP. Therefore, it is suggested that this process does not require energy and does not involve the tonoplast ATPase. The ATP-dependent arginine uptake was strongly inhibited by p-chloromercuriphenylsulfonic acid. Furthermore, hydrophobic amino acids were inhibitory (I50 phenylalanine 1 millimolar). Similar characteristics were observed for the uptake of aspartic acid. However, rates of ATP-stimulated aspartic acid transport were 10-fold lower as compared to arginine transport. Uptake of aspartate in the absence of ATP was negligible.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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