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. 1991 Oct;97(2):832–835. doi: 10.1104/pp.97.2.832

Hygromycin Resistance Gene Cassettes for Vector Construction and Selection of Transformed Rice Protoplasts 1

Zhenwei Zheng 1, Akio Hayashimoto 1,2, Zhijian Li 1, Norimoto Murai 1
PMCID: PMC1081085  PMID: 16668477

Abstract

Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Pstl, and Sacll sites in the coding sequence of a hygromycin B phosphotransferase gene (hph) from Escherichia coli were eliminated. The mutated hph genes were used to form gene cassettes flanked by EcoRI-Sacll-Kpnl-Hindlll sites. Hygromycin resistance of wild-type and mutated hph genes was indistinguishable in E. coli and rice protoplast growth assay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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