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. 1991 Nov;97(3):928–935. doi: 10.1104/pp.97.3.928

Energy Status and Functioning of Phosphorus-Deficient Soybean Nodules 1

Tong-Min Sa 1,2,3, Daniel W Israel 1,2,3
PMCID: PMC1081106  PMID: 16668533

Abstract

Characterization of the effects of long-term P deficiency and of onset and recovery from P deficiency on bacteroid mass and number per unit nodule mass and energy status of soybean (Glycine max L. Merr.) nodules was used to investigate the mechanisms by which P deficiency decreases symbiotic N2 fixation. The continuous P deficiency treatment (0.05 millimolar P) significantly decreased the whole plant dry mass, P, and N by 62, 90, and 78%, respectively, relative to the P-sufficient control (1.0 millimolar) at 44 days after transplanting. Specific nitrogenase activity was decreased an average of 28% over a 16-day experimental period by P deficiency. Whole nodules of P-deficient controls contained 70 to 75% lower ATP concentrations than nodules of P-sufficient controls. Energy charge and ATP concentrations in the bacteroid fraction of nodules were not significantly affected by P treatment. However, ATP and total adenylate concentrations and energy charge in the plant cell fraction of nodules were significantly decreased 91, 62, and 50%, respectively, by the P deficiency treatment. Specific nitrogenase activity, energy charge, and ATP concentration in the plant cell fraction increased to the levels of nonstressed controls within 2, 2, and 4 days, respectively, after alleviation of external P limitation, whereas bacteroid mass per unit nodule mass and bacteroid N concentration did not increase to the level of nonstressed controls until 7 days after alleviation of external P limitation. All of these parameters except bacteroid mass per unit nodule mass decreased to the levels of the P-deficient controls by 11 days after onset of external P limitation. Concentration of ATP in the bacteroid fraction was not significantly affected by alteration in the external P supply. Energy charge in the bacteroid fraction from plants recovering from P deficiency was decreased to a small (10%) but significant extent (P < 0.05) at two sampling dates relative to P-sufficient controls. These ATP concentration and energy charge measurements indicate that P deficiency impaired oxidative phosphorylation in the plant cell fraction of nodules to a much greater extent than in the bacteroids. The concurrence of significant changes in specific nitrogenase activity (2 days) and in the energy charge (2 days) and ATP concentration (4 days) in the plant cell fraction during recovery from external P limitation is consistent with the conclusion that P deficiency decreases the specific nitrogenase activity by inhibiting an energy-dependent reaction(s) in the plant cell fraction of the nodules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chapman A. G., Fall L., Atkinson D. E. Adenylate energy charge in Escherichia coli during growth and starvation. J Bacteriol. 1971 Dec;108(3):1072–1086. doi: 10.1128/jb.108.3.1072-1086.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ching T. M., Hedtke S., Russell S. A., Evans H. J. Energy State and Dinitrogen Fixation in Soybean Nodules of Dark-grown Plants. Plant Physiol. 1975 Apr;55(4):796–798. doi: 10.1104/pp.55.4.796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ching T. M. Regulation of nitrogenase activity in soybean nodules by ATP and energy charge. Life Sci. 1976 May 15;18(10):1071–1076. doi: 10.1016/0024-3205(76)90140-5. [DOI] [PubMed] [Google Scholar]
  4. Daesch G., Mortenson L. E. Sucrose catabolism in Clostridium pasteurianum and its relation to N2 fixation. J Bacteriol. 1968 Aug;96(2):346–351. doi: 10.1128/jb.96.2.346-351.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gallon J. R., LaRue T. A., Kurz W. G. Characteristics of nitrogenase activity in broken cell preparations of the blue-green alga Gloeocapsa sp. LB 795. Can J Microbiol. 1972 Mar;18(3):327–332. doi: 10.1139/m72-050. [DOI] [PubMed] [Google Scholar]
  6. Israel D. W. Investigation of the role of phosphorus in symbiotic dinitrogen fixation. Plant Physiol. 1987 Jul;84(3):835–840. doi: 10.1104/pp.84.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Israel D. W., Jackson W. A. Ion balance, uptake, and transport processes in n(2)-fixing and nitrate- and urea-dependent soybean plants. Plant Physiol. 1982 Jan;69(1):171–178. doi: 10.1104/pp.69.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaluza K., Hennecke H. Regulation of nitrogenase messenger RNA synthesis and stability in Klebsiella pneumoniae. Arch Microbiol. 1981 Sep;130(1):38–43. doi: 10.1007/BF00527069. [DOI] [PubMed] [Google Scholar]
  9. Khym J. X. An analytical system for rapid separation of tissue nucleotides at low pressures on conventional anion exchangers. Clin Chem. 1975 Aug;21(9):1245–1252. [PubMed] [Google Scholar]
  10. Mathis J. N., Israel D. W., Barbour W. M., Jarvis B. D., Elkan G. H. Analysis of the Symbiotic Performance of Bradyrhizobium japonicum USDA 110 and Its Derivative I-110 and Discovery of a New Mannitol-Utilizing, Nitrogen-Fixing USDA 110 Derivative. Appl Environ Microbiol. 1986 Jul;52(1):75–80. doi: 10.1128/aem.52.1.75-80.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McClure P. R., Israel D. W. Transport of nitrogen in the xylem of soybean plants. Plant Physiol. 1979 Sep;64(3):411–416. doi: 10.1104/pp.64.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McParland R. H., Guevara J. G., Becker R. R., Evans H. J. The purification and properties of the glutamine synthetase from the cytosol of Soya-bean root nodules. Biochem J. 1976 Mar 1;153(3):597–606. doi: 10.1042/bj1530597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shanmugam K. T., Valentine R. C. Molecular biology of nitrogen fixation. Science. 1975 Mar 14;187(4180):919–924. doi: 10.1126/science.238283. [DOI] [PubMed] [Google Scholar]
  14. Sloger C. Symbiotic effectiveness and n(2) fixation in nodulated soybean. Plant Physiol. 1969 Dec;44(12):1666–1668. doi: 10.1104/pp.44.12.1666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Streeter J. G. Estimation of ammonium concentration in the cytosol of soybean nodules. Plant Physiol. 1989 Jul;90(3):779–782. doi: 10.1104/pp.90.3.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weisz P. R., Denison R. F., Sinclair T. R. Response to drought stress of nitrogen fixation (acetylene reduction) rates by field-grown soybeans. Plant Physiol. 1985 Jul;78(3):525–530. doi: 10.1104/pp.78.3.525. [DOI] [PMC free article] [PubMed] [Google Scholar]

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