Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1991 Nov;97(3):1256–1259. doi: 10.1104/pp.97.3.1256

Effect of Hydrogen Cyanamide on Amino Acid Profiles in Kiwifruit Buds during Budbreak

Eric F Walton 1, Christopher J Clark 1, Helen L Boldingh 1
PMCID: PMC1081151  PMID: 16668518

Abstract

Buds, and resultant shoots, were collected from kiwifruit (Actinidia deliciosa [A. Chev.] CF Liang et AR Ferguson var deliciosa cv Hayward) vines from late autumn until late spring with and without hydrogen cyanamide treatment. Those samples were weighed and analyzed for total nitrogen and free amino acids. By 7 days after hydrogen cyanamide treatment, the amount of proline had risen to nearly one-quarter of the total amino acid pool in the treated buds and that proportion was maintained for at least 14 days before it declined. The maximum concentration detected in treated buds was 25.8 micromoles per gram dry weight, 6.6 times that detected in untreated buds. By 95 days after treatment, the relative amounts of proline were not significantly different.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bieleski R. L., Turner N. A. Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chromatography. Anal Biochem. 1966 Nov;17(2):278–293. doi: 10.1016/0003-2697(66)90206-5. [DOI] [PubMed] [Google Scholar]
  2. Eggleston L. V., Krebs H. A. Regulation of the pentose phosphate cycle. Biochem J. 1974 Mar;138(3):425–435. doi: 10.1042/bj1380425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kohl D. H., Lin J. J., Shearer G., Schubert K. R. Activities of the pentose phosphate pathway and enzymes of proline metabolism in legume root nodules. Plant Physiol. 1990 Nov;94(3):1258–1264. doi: 10.1104/pp.94.3.1258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kohl D. H., Schubert K. R., Carter M. B., Hagedorn C. H., Shearer G. Proline metabolism in N2-fixing root nodules: energy transfer and regulation of purine synthesis. Proc Natl Acad Sci U S A. 1988 Apr;85(7):2036–2040. doi: 10.1073/pnas.85.7.2036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Redgwell R. J. Fractionation of plant extracts using ion-exchange Sephadex. Anal Biochem. 1980 Sep 1;107(1):44–50. doi: 10.1016/0003-2697(80)90489-3. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES