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. 1991 Dec;97(4):1585–1587. doi: 10.1104/pp.97.4.1585

Efficiency of Particle-Bombardment-Mediated Transformation Is Influenced by Cell Cycle Stage in Synchronized Cultured Cells of Tobacco 1

Asako Iida 1,2,3, Toshiya Yamashita 1,2,3, Yasuyuki Yamada 1,2,3, Hiromichi Morikawa 1,2,3
PMCID: PMC1081205  PMID: 16668589

Abstract

Plasmid DNA pB1221 harboring β-glucuronidase gene was delivered to synchronized cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells of different cell cycle stages by a pneumatic particle gun. The cells bombarded at M and G2 phases gave 4 to 6 times higher transformation efficiency than those bombarded at the S and G1 phases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Yamashita T., Iida A., Morikawa H. Evidence That More than 90% of beta-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus. Plant Physiol. 1991 Oct;97(2):829–831. doi: 10.1104/pp.97.2.829. [DOI] [PMC free article] [PubMed] [Google Scholar]

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