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. 2024 Jan 20;12(1):215. doi: 10.3390/microorganisms12010215

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Macroscopic and microscopic features of Methanobrevibacter massiliense. (a) Macroscopic observation of M. massiliense and P. piscolens co-cultures, dark deposits are visible. (b) Electron microscopy (TM4000 HITACHI, 10 KV, × 1.500), coccobacilli are visible, but no morphologic features made it possible to distinguish between M. massiliense and P. piscolens. (c) Confocal microscopy merged view of autofluorescence at 420 nm and brightfill mode; autofluorescent coccobacilli are visible as well as non-autofluorescent coccobacilli (LSM 900 (Carl Zeiss Microscopy GmbH)).