Skip to main content
. 2024 Jan 19;16(2):307. doi: 10.3390/nu16020307

Figure 2.

Figure 2

Characterization of AFPE components: (a) Elution profile of AFPE components after gel-filtration chromatography (HiLoad Superdex 75™ 16/60 column) monitored at 280 nm. V0 and Vi indicate the void and inclusion volumes of the gel-filtration column, respectively. (b) SDS-PAGE analysis of Peaks 1–4 after gel-filtration separation. Lane 1: M, molecular weight marker; Lane 2: AFPE; Lanes 3, 4, 5, and 6: aliquots of eluted fractions (34, 35, 36, and 37, respectively) from Peak 1. Lanes 7, 8, and 9: aliquots of Peaks 2, 3, and 4, respectively. SDS-PAGE was carried out with a 15% polyacrylamide separating gel and stained with Coomassie blue; the arrow shows the ~33 kDa protein.