Extended Data Fig. 1 |. FezB plays a moderate role in protection against oxidative stress.

(a) The growth kinetics of C. difficile WT and ΔfezB grown in BHIS with or without treatment of 1 mM FeSO₄. (b) Cells were sub-cultured in BHIS and inoculated in BHIS amended with varied concentrations of H₂O₂. (c) Cells were sub-cultured in BHIS supplemented with 100 μM dipyridyl and inoculated in BHIS amended with 100 μM dipyridyl plus varied concentrations of H₂O₂. Experiments were conducted three times independently with similar results. Data are presented as mean ± STD (n = 3).