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. 2024 Jan 4;27(2):108795. doi: 10.1016/j.isci.2024.108795

Figure 4.

Figure 4

Toll-1 but not Toll-7 activated autophagy is dMyd88 dependent

(A‒D) Detection of LC3-I, LC3-II, and P62 proteins in S2 cells treated with dsRNAs for dMyd88, Tube, Pelle, and Cactus by immunoblotting. S2 cells stably expressing RFP-GFP-LC3 were cultured in complete medium without treatment (control), or cultured in PBS instead of complete medium for 6 h (starved), or co-transfected with pMT-TIR-1-V5 or pMT-TIR-7-V5 with dsRNA for GFP (dsGFP), dMyd88 (dsMyd88) (A), Tube (dsTube) (B), Pelle (dsPelle) (C), or Cactus (dsCactus) (D), expression of TIR-1-V5 and TIR-7-V5 was detected by immunoblotting with mouse anti-V5 monoclonal antibody, LC3-I and LC3-II in these S2 cells were detected by rabbit anti-LC3 polyclonal antibody, P62 protein was detected by anti-Ref(2)P antibody, while tubulin was detected by mouse anti-tubulin monoclonal antibody. Data were represented as means ± SEM. For determination of relative protein level and significant differences, see Figure 1 legend.