A) Flowchart of snRNA-seq experiment. Flies are collected at 30, 50, and 70 days. The heads and bodies of males and females are processed separately. 5d samples are from the FCA.
B) Number of nuclei collected from different ages and sexes.
C) tSNE visualizations of the head and body samples from different ages.
D) tSNE visualizations showing broad cell classes of datasets integrated across different time points.
E) Number of nuclei for each broad cell class shown in 1D.