Figure 5.

Embigin deficiency leads to compromised lung function

(A) Heatmap of all expressed genes in WT (n = 5) and Emb^−/− (n = 5) lung and placenta at E17.5 based on RNA sequencing analysis. The genes have been sorted from smallest to largest fold change value of Emb^−/− vs. WT comparison and visualized by Morpheus.

(B) Differentially expressed genes in WT (n = 5) vs. Emb^−/− embryonic lungs (n = 5) at E17.5 were analyzed from RNA sequencing data by WebGestalt. Top19 of the most enriched biological processes of those with FDR <0.05 and ≥3 overlapping genes are shown.

(C) WT and Emb^−/− lung paraffin sections were immunostained with Ki67 antibody at E17.5. Nuclei were labeled with Hoechst 33342 Fluorescent Stain. Scale bars: 100 μm. Ki67-positive cells in WT (n = 5) and Emb^−/− (n = 5) embryonic lungs at E17.5 were analyzed as fractions of all cells from five different areas of each lung. Statistical significance (p = 0.0002) was determined by the Student’s T-test for independent samples. Data are represented as a Spear style boxplot.

(D) Embigin mRNA expression in developing lungs from E10.5 to E16.5 based on the analysis of scRNA-seq data published in the MOSTA database.

See also Figures S4 and S6.