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. Author manuscript; available in PMC: 2024 Dec 7.
Published in final edited form as: Cell. 2023 Nov 27;186(25):5569–5586.e21. doi: 10.1016/j.cell.2023.10.024

Figure 6. Human genome-wide TScan-II screens of clonally expanded CD4+ T-cells in SjD.

Figure 6

A) Schematic representation of the experimental workflow to characterize clonally expanded CD4+ T-cell clones in SjD patients.

B-E) TScan-II screens were performed against the human genome-wide v2 library with eHEK-293-II cells expressing HLA-DRB1*04, HLA-DRB1*03:01, and HLA-DQB1*02_HLA-DQA1*05. B) Pooled TScan-II screen of 5 TCRs 4A,7A,7G,9B, and 10C C) TScan-II screen of 4A TCR. D) Pooled TScan-II screen using 5 TCRs 3A,10A1,10D,10E, and 10F. E) Pooled TScan-II using 6 TCRs 3A,4A,9C,8A,8B and 5B.

F) FACS plots depicting activation of GzB reporter following a 6 h co-culture experiment with 3A and 4A centaur T-cells towards the indicated cognate antigens.

G) Relative activation of the GzB reporter in cells pulsed with the indicated peptide fragment cocultured with 4A T-cells.