Abstract
Growth of the excised axis of Phaseolus vulgaris L. (var. White Marrowfat) begins after a 7-hour incubation in buffer or water at 26°. Growth, as measured by axis elongation or fresh weight increase, is linear for at least 8 hours with a resultant fresh weight increase of approximately 65%. Cell elongation begins 4 or 5 hours prior to cell division and 5 or 6 hours prior to radicle protrusion in the intact seed.
The initiation of axis elongation is apparently dependent on synthesis of RNA and protein. Both actinomycin D and puromycin inhibit the initiation of elongation. Actinomycin I) inhibits the incorporation of ATP-8-C14 into axis RNA and C14-leucine into protein, while puromycin inhibits the incorporation of C14-leucine into axis protein.
The respiratory rate of the axes increases sharply at about the time of initiation of cell elongation. Dinitrophenol initially increases O2 uptake by the axes, but at the end of 15 hours the rates of O2 uptake by control or dinitrophenol-treated axes are approximately the same.
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Selected References
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- Key J. L., Ingle J. REQUIREMENT FOR THE SYNTHESIS OF DNA-LIKE RNA FOR GROWTH OF EXCISED PLANT TISSUE. Proc Natl Acad Sci U S A. 1964 Dec;52(6):1382–1388. doi: 10.1073/pnas.52.6.1382. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolff S. Some Aspects of the Chemical Protection against Radiation Damage to Vicia Faba Chromosomes. Genetics. 1954 May;39(3):356–364. doi: 10.1093/genetics/39.3.356. [DOI] [PMC free article] [PubMed] [Google Scholar]