Skip to main content
Plant Physiology logoLink to Plant Physiology
. 1966 Mar;41(3):383–388. doi: 10.1104/pp.41.3.383

DNA From Plant Mitochondria 1

Y Suyama 1,2, W D Bonner Jr 1,2
PMCID: PMC1086353  PMID: 16656268

Abstract

DNA was isolated from a mitochondrial fraction of each of the following plant materials: Mung bean (Phaseolus aureus) etiolated hypocotyl; turnip (Brassica rapa) root; sweet potato (Ipomoea batatas) root; and onion (Allium cepa) bulb. It was found that all of these mitochondrial fractions contained DNA, the densities of which were identical (ρ=1.706 g·cm−3). An additional DNA (ρ=1.695) band found in the mitochondrial fraction of Brassica rapa, was identical to DNA separately isolated from the chloroplast-rich fraction. The origin of the second DNA from Allium mitochondrial fraction was not identified.

Contrary to the identity of the mitochondrial DNA, DNA from nuclear fractions differed not only with each other but from the corresponding mitochondrial DNA.

DNA from Phaseolus and Brassica mitochondria showed the hyperchromicity characteristic of double stranded, native DNA upon heating; Tm's in 0.0195 Na+ were the same; 72.0°. The amount of DNA within the mitochondrion of Phaseolus was estimated to be 5.0 × 10−10 μg; this estimate was made by isolating the mitochondrial DNA concomitantly with the known amount of added 15N2H B. subtilis DNA (ρ=1.740). Approximately the same amount of DNA was present in the mitochondrion of Brassica or Ipomoea.

Full text

PDF
383

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. LUCK D. J. Formation of mitochondria in Neurospora crassa. A quantitative radioautographic study. J Cell Biol. 1963 Mar;16:483–499. doi: 10.1083/jcb.16.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  3. SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
  4. SUEOKA N. On the genetic basis of variation and heterogeneity of DNA base composition. Proc Natl Acad Sci U S A. 1962 Apr 15;48:582–592. doi: 10.1073/pnas.48.4.582. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES