Abstract
Acetate was incorporated by Chlorella pyrenoidosa into nonvolatile water-soluble compounds (amino acids, organic acids and carbohydrates) with pH optimum between 4 and 5. Incorporation into lipid was maximal at pH 7.5. The proportion of incorporated acetate in lipid was not significantly affected by acetate concentration and chlorophyll concentration in the ranges tested. Illumination of Chlorella during acetate metabolism increased the synthesis of lipid with concomitant decrease in the synthesis of water-soluble compounds. Nitrate and ammonium ions had essentially no effect on acetate metabolism. Inhibition by nitrite was greater on the synthesis of lipid. Illumination reversed the gross inhibition by nitrite, but altered the distribution of incorporated label in favor of the water-soluble compounds.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- Erickson L. C., Wedding R. T., Brannaman B. L. Influence of pH On 2,4-Dichlorophenoxyacetic and Acetic Acid Activity in Chlorella. Plant Physiol. 1955 Jan;30(1):69–74. doi: 10.1104/pp.30.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MUDD J. B., McMANUS T. T. Metabolism of acetate by cellfree preparations from spinach leaves. J Biol Chem. 1962 Jul;237:2057–2063. [PubMed] [Google Scholar]
- PANEQUE A., RAMIREZ J. M., DELCAMPO F. F., LOSADA M. LIGHT AND DARK REDUCTION OF NITRITE IN A RECONSTITUTED ENZYMIC SYSTEM. J Biol Chem. 1964 Jun;239:1737–1741. [PubMed] [Google Scholar]
- STUMPF P. K., JAMES A. T. The biosynthesis of long-chain fatty acids by lettuce chloroplast preparations. Biochim Biophys Acta. 1963 Feb 19;70:20–32. doi: 10.1016/0006-3002(63)90715-7. [DOI] [PubMed] [Google Scholar]
- Spoehr H. A., Milner H. W. THE CHEMICAL COMPOSITION OF CHLORELLA; EFFECT OF ENVIRONMENTAL CONDITIONS. Plant Physiol. 1949 Jan;24(1):120–149. doi: 10.1104/pp.24.1.120. [DOI] [PMC free article] [PubMed] [Google Scholar]