Abstract
Whole cell difference spectra of the blue-green algae, Saprospira grandis, Leucothrix mucor, and Vitreoscilla sp. have one, or at the most 2, broad α-bands near 560 mμ. At −190° these bands split to give 4 peaks in the α-region for b and c-type cytochromes, but no α-band for a-type cytochromes is visible. The NADH oxidase activity of these organisms was shown to be associated with particulate fractions of cell homogenates. The response of this activity to inhibitors differed from the responses of the NADH oxidase activities of particulate preparations from the green algae and higher plants to the same inhibitors, but is more typical of certain bacteria. No cytochrome oxidase activity was present in these preparations. The respiration of Saprospira and Vitreoscilla can be light-reversibly inhibited by CO, and all 3 organisms have a CO-binding pigment whose CO complex absorbs near 570, 535, and 417 mμ. The action spectrum for the light reversal of CO-inhibited Vitreoscilla respiration shows maxima at 568, 534, and 416 mμ. The results suggest that the terminal oxidase in these blue-greens is an o-type cytochrome.
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- ORLANDO J. A., LEVINE L., KAMEN M. D. Immunochemical studies on Rhodospirillum haem protein. Biochim Biophys Acta. 1961 Jan 1;46:126–133. doi: 10.1016/0006-3002(61)90655-2. [DOI] [PubMed] [Google Scholar]
- PACKER L. Respiratory carriers involved in the oxidation of hydrogen and lactate in a facultative autotroph. Arch Biochem Biophys. 1958 Nov;78(1):54–65. doi: 10.1016/0003-9861(58)90314-x. [DOI] [PubMed] [Google Scholar]
- TANIGUCHI S., KAMEN M. D. THE OXIDASE SYSTEM OF HETEROTROPHICALLY-GROWN RHODOSPIRILLUM RUBRUM. Biochim Biophys Acta. 1965 Mar 22;96:395–428. doi: 10.1016/0005-2787(65)90560-5. [DOI] [PubMed] [Google Scholar]
- WHITE D. C., SMITH L. Hematin enzymes of Hemophilus parainfluenzae. J Biol Chem. 1962 Apr;237:1332–1336. [PubMed] [Google Scholar]
- Webster D. A., Hackett D. P. Respiratory Chain of Colorless Algae I. Chlorophyta and Euglenophyta. Plant Physiol. 1965 Nov;40(6):1091–1100. doi: 10.1104/pp.40.6.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]