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. 1966 Jun;41(6):919–922. doi: 10.1104/pp.41.6.919

Effects of Protein Inhibitors and Auxin on Nucleic Acid Metabolism in Peanut Cotyledons

Walter J G Carpenter 1, Joe H Cherry 1
PMCID: PMC1086452  PMID: 16656356

Abstract

The accumulation of labeled phosphorus into newly synthesized nucleic acids or peanut cotyledon slices incubated with chloramphenicol, puromycin, or 2,4-dichlorophenoxyacetic acid (2,4-D) was reduced. Promotion of nucleic acid synthesis was not noted by any of these chemicals. Chloramphenicol completely inhibited the synthesis of the DNA-RNA fraction at 1.25 × 10−3 m while soluble and ribosomal RNA was inhibited by 70% and 80%, respectively. At the same concentration messenger RNA was inhibited by only 40%. These effects suggest that chloramphenicol inhibit nucleic acid synthesis in peanut cotyledons in a differential manner. Similar results were noted for DNA at low concentrations of 2,4-D. However, at high concentrations of 2,4-D, DNA as well as RNA fractions were inhibited in a similar manner at a given concentration. Puromycin did not differentially inhibit nucleic acid synthesis except at 2 × 10−3 m where DNA was least inhibited.

Nondifferential inhibition suggests that a common site or precursor pool essential for the synthesis of all nucleic acid fractions is altered. Differential inhibition may be due to the interference with a specific rate-limiting step, directly or indirectly, in the formation of a particular nucleic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN D. W., ZAMECNIK P. C. The effect of puromycin on rabbit reticulocyte ribosomes. Biochim Biophys Acta. 1962 Jun 11;55:865–874. doi: 10.1016/0006-3002(62)90899-5. [DOI] [PubMed] [Google Scholar]
  2. ARONSON A. I., SPIEGELMAN S. Protein and ribonucleic acid synthesis in a chloramphenicol-inhibited system. Biochim Biophys Acta. 1961 Oct 14;53:70–84. doi: 10.1016/0006-3002(61)90795-8. [DOI] [PubMed] [Google Scholar]
  3. CANNON M., KRUG R., GILBERT W. THE BINDING OF S-RNA BY ESCHERICHIA COLI RIBOSOMES. J Mol Biol. 1963 Oct;7:360–378. doi: 10.1016/s0022-2836(63)80030-3. [DOI] [PubMed] [Google Scholar]
  4. CHERRY J. H. ASSOCIATION OF RAPIDLY METABOLIZED DNA AND RNA. Science. 1964 Nov 20;146(3647):1066–1069. doi: 10.1126/science.146.3647.1066. [DOI] [PubMed] [Google Scholar]
  5. Cherry J. H., Chroboczek H., Carpenter W. J., Richmond A. Nucleic Acid Metabolism in Peanut Cotyledons. Plant Physiol. 1965 May;40(3):582–587. doi: 10.1104/pp.40.3.582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cherry J. H. Nucleic Acid, Mitochondria, & Enzyme Changes in Cotyledons of Peanut Seeds during Germination. Plant Physiol. 1963 Jul;38(4):440–446. doi: 10.1104/pp.38.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GALE E. F., FOLKES J. P. The assimilation of amino-acids by bacteria. XV. Actions of antibiotics on nucleic acid and protein synthesis in Staphylococcus aureus. Biochem J. 1953 Feb;53(3):493–498. doi: 10.1042/bj0530493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HOLLAND J. J. EFFECTS OF PUROMYCIN ON RNA SYNTHESIS IN MAMMALIAN CELLS. Proc Natl Acad Sci U S A. 1963 Sep;50:436–446. doi: 10.1073/pnas.50.3.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KURLAND C. G., MAALOE O. Regulation of ribosomal and transfer RNA synthesis. J Mol Biol. 1962 Mar;4:193–210. doi: 10.1016/s0022-2836(62)80051-5. [DOI] [PubMed] [Google Scholar]
  10. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  11. NATHANS D. PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS: INCORPORATION OF PUROMYCIN INTO PEPTIDE CHAINS. Proc Natl Acad Sci U S A. 1964 Apr;51:585–592. doi: 10.1073/pnas.51.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SPECTOR A., KINOSHITA J. H. THE EFFECT OF ACTINOMYCIN D AND PUROMYCIN UPON RNA AND PROTEIN METABOLISM IN CALF LENS. Biochim Biophys Acta. 1965 Apr 19;95:561–568. doi: 10.1016/0005-2787(65)90510-1. [DOI] [PubMed] [Google Scholar]
  13. TAMAOKI T., MUELLER G. C. Effect of puromycin on RNA synthesis in HeLa cells. Biochem Biophys Res Commun. 1963 May 28;11:404–410. doi: 10.1016/0006-291x(63)90131-1. [DOI] [PubMed] [Google Scholar]
  14. YANKOFSKY S. A., SPIEGELMAN S. Distinct cistrons for the two ribosomal RNA components. Proc Natl Acad Sci U S A. 1963 Apr;49:538–544. doi: 10.1073/pnas.49.4.538. [DOI] [PMC free article] [PubMed] [Google Scholar]

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